Function of cryopreserved pig aortas

• Published in: The Journal of surgical research 2004-08, Vol.120 (2), p.304-311
• Main Authors: Rendal, E., Rodrı́guez, M., Martı́nez, M.V., Fernández, R.O., Sánchez, J., Segura, R., Bermúdez, T., Matheu, G., Filgueira, P., Pértega, S., Andión, C.
• Format: Article
• Language: English
• Subjects: Animals; Aorta - pathology; Aorta - physiopathology; Biological and medical sciences; contraction; Cryopreservation; Endothelium, Vascular - pathology; Endothelium, Vascular - physiopathology; functional activity; General aspects; Medical sciences; relaxation; Swine; vascular segments; Vasoconstriction; Vasodilation
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2004.02.004

This paper analyzes the influence of storage in the gas phase or liquid phase on grafts, together with the thawing method (15°C/min or 100°C/min) on the postthawing activity of pig cryopreserved arterial grafts (aortas). Obtainment of arterial grafts (aortas) was from pigs with an ischemic time not greater than 2 h. Each aorta was divided into five fragments and assigned randomly to one control group of fresh aorta and four groups of cryopreserved aortas: group 1: gas phase/slow thawing; group 2: gas phase/rapid thawing; group 3: liquid phase/slow thawing; and group 4: liquid phase/rapid thawing. After the incubation in antibiotic solution, the cryopreservation in RPMI medium +10% DMSO was carried out and the level of cooling used was a reduction of 1°C/min. The contraction and relaxation responses of the fresh and frozen/thawed arteries were carried out in organ baths. After thawing, the sensitivity to various agonists and maximal responses to the endothelium-dependent and independent relaxant agents were decreased. The maximal responses to the tested vasoconstrictors (KCl and noradrenaline) were, respectively, 13% and 24% of the responses obtained in unfrozen aortas. The endothelium-independent relaxant responses to sodium nitroprusside (SNP) were reduced and important reductions of the endothelium-dependent relaxant responses to acetylcholine were produced. The cryopreservation of pig aortas under the conditions used in this study led to a decrease in the contractility of the pig aortas, as well as a decrease in the endothelium-independent relaxant responses. On the other hand, no apparent preservation of the endothelium-dependent relaxant responses was observed.