Functional assessment of cryopreserved human aortas for pharmaceutical research

We evaluated the impact of standard cryopreservation on functional properties of human aortic homografts. From seven human donors, the thoracic descending aorta was obtained. Effects of cryopreservation on contractibility and endothelium function were tested. After cryopreservation no endothelium-de...

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Bibliographic Details
Published in:Cell and tissue banking 2004, Vol.5 (2), p.119-123
Main Authors: Rendal Vázquez, Maria Esther, Rodríguez Cabarcos, M, Martínez Santos, M V, Fernández Mallo, R O, Sánchez Ibáñez, J, Segura Iglesias, R, Matheu Capó, G, Filgueira Fernández, P, Pértega Díaz, S, Bermúdez González, T, Andión Núñez, C
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Aorta, Thoracic - drug effects
Aorta, Thoracic - physiology
Cryopreservation
Endothelium, Vascular - drug effects
Endothelium, Vascular - physiology
Female
Humans
In Vitro Techniques
Male
Middle Aged
Nitroprusside - pharmacology
Norepinephrine - pharmacology
Tissue Preservation
Vasoconstriction - drug effects
Vasodilation - drug effects
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Summary:We evaluated the impact of standard cryopreservation on functional properties of human aortic homografts. From seven human donors, the thoracic descending aorta was obtained. Effects of cryopreservation on contractibility and endothelium function were tested. After cryopreservation no endothelium-dependent or endothelium-independent relaxation was found and the contractibility was strongly affected. Arteries showed no function and loss of endothelial integrity after cryopreservation and thawing.
ISSN:1389-9333
1573-6814
DOI:10.1023/B:CATB.0000034087.26468.5a