Production of Bioactive Cellulose Films Reconstituted from Ionic Liquids

A new method for introducing enzymes into cellulosic matrixes which can be formed into membranes, films, or beads has been developed using a cellulose-in-ionic-liquid dissolution and regeneration process. Initial results on the formation of thin cellulose films incorporating dispersed laccase indica...

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Bibliographic Details
Published in:Biomacromolecules 2004-07, Vol.5 (4), p.1379-1384
Main Authors: Turner, Megan B, Spear, Scott K, Holbrey, John D, Rogers, Robin D
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Biotechnology
Catalysis
Cellulose - analogs & derivatives
Cellulose - chemical synthesis
Cellulose - chemistry
Cellulose and derivatives
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Ions - chemistry
Laccase - chemistry
Membranes, Artificial
Methods. Procedures. Technologies
Molecular Structure
Natural polymers
Oxidation-Reduction
Physicochemistry of polymers
Solvents - chemistry
Surface Properties
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Summary:A new method for introducing enzymes into cellulosic matrixes which can be formed into membranes, films, or beads has been developed using a cellulose-in-ionic-liquid dissolution and regeneration process. Initial results on the formation of thin cellulose films incorporating dispersed laccase indicate that active enzyme-encapsulated films can be prepared using this methodology and that precoating the enzyme with a second, hydrophobic ionic liquid prior to dispersion in the cellulose/ionic liquid solution can provide an increase in enzyme activity relative to that of untreated films, presumably by providing a stabilizing microenvironment for the enzyme.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm049748q