Short-Term Caloric Restriction and Sites of Oxygen Radical Generation in Kidney and Skeletal Muscle Mitochondria

: Mitochondrial free radical generation is believed to be one of the principal factors determining aging rate, and complexes I and III have been described as the main sources of reactive oxygen species (ROS) within mitochondria in heart, brain, and liver. Moreover, complex I ROS generation of heart...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2004-06, Vol.1019 (1), p.333-342
Main Authors: GREDILLA, RICARDO, PHANEUF, SHARON, SELMAN, COLIN, KENDAIAH, SUMA, LEEUWENBURGH, CHRISTIAAN, BARJA, GUSTAVO
Format: Article
Language:English
Subjects:
Aging
Animals
Caloric Restriction
Electron Transport
Free Radicals
Hydrogen Peroxide - pharmacology
Kidney - metabolism
Longevity
Male
mitochondria
Mitochondria - pathology
Muscle, Skeletal - metabolism
Oxygen - metabolism
Oxygen Consumption
Rats
Rats, Inbred F344
Reactive Oxygen Species
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Summary:: Mitochondrial free radical generation is believed to be one of the principal factors determining aging rate, and complexes I and III have been described as the main sources of reactive oxygen species (ROS) within mitochondria in heart, brain, and liver. Moreover, complex I ROS generation of heart and liver mitochondria seems especially linked to aging rate both in comparative studies between animals with different longevities and in caloric restriction models. Caloric restriction (CR) is a well‐documented manipulation that extends mean and maximum longevity. One of the factors that appears to be involved in such life span extension is the reduction in mitochondrial free radical generation at complex I. We have performed two parallel investigations, one studying the effect of short‐term CR on oxygen radical generation in kidney and skeletal muscle (gastrocnemius) mitochondria and a second one regarding location of mitochondrial ROS‐generating sites in these same tissues. In the former study, no effect of short‐term caloric restriction was observed in mitochondrial free radical generation in either kidney or skeletal muscle. The latter study ruled out complex II as a principal source of free radicals in kidney and in skeletal muscle mitochondria, and, similar to previous investigations in heart and liver organelles, the main free radical generators were located at complexes I and III within the electron transport system.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1297.057