Multiple Targets for Suppression of RNA Interference by Tomato Aspermy Virus Protein 2B

Viral suppressors of RNA interference (RNAi) appear to have evolved as a response to this innate genomic defense. We report the nucleic acid binding properties of the Cucumovirus RNAi suppressor tomato aspermy virus protein 2B (TAV 2B). Using total internal reflection fluorescence spectroscopy (TIRF...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Easton) 2008-12, Vol.47 (48), p.12655-12657
Main Authors: Rashid, Umar Jan, Hoffmann, Jan, Brutschy, Bernhard, Piehler, Jacob, Chen, Julian C.-H
Format: Article
Language:English
Subjects:
Cucumovirus - genetics
Cucumovirus - metabolism
Lycopersicon esculentum - virology
Oligoribonucleotides - genetics
Oligoribonucleotides - metabolism
Peptide Fragments - metabolism
Protein Binding
RNA Interference - physiology
RNA, Viral - genetics
RNA, Viral - metabolism
Spectrometry, Fluorescence
Viral Proteins - genetics
Viral Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Viral suppressors of RNA interference (RNAi) appear to have evolved as a response to this innate genomic defense. We report the nucleic acid binding properties of the Cucumovirus RNAi suppressor tomato aspermy virus protein 2B (TAV 2B). Using total internal reflection fluorescence spectroscopy (TIRFS), we show that TAV 2B binds double-stranded RNA corresponding to siRNAs and miRNAs, as well as single-stranded RNA oligonucleotides. A number of positively charged residues between amino acids 20 and 30 are critical for RNA binding. Binding to RNA oligomerizes and induces a conformational change in TAV 2B, causing it to form a primarily helical structure and a 4:2 protein−RNA complex.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi801281h