Ontogenetic profile of glutamate uptake in brain structures slices from rats: sensitivity to guanosine

The excitotoxicity of the neurotransmitter glutamate has been shown to be connected with many acute and chronic diseases of the CNS. High affinity sodium-dependent glutamate transporters play a key role in maintaining adequate levels of extracellular glutamate. In the present study, we used slices o...

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Bibliographic Details
Published in:Mechanisms of ageing and development 2004-07, Vol.125 (7), p.475-481
Main Authors: Thomazi, Ana Paula, Godinho, Graça F.R.S, Rodrigues, Juliana M, Schwalm, Fábio D, Frizzo, Marcos E.S, Moriguchi, Emı́lio, Souza, Diogo O, Wofchuk, Susana T
Format: Article
Language:English
Subjects:
Ageing
Aging - physiology
Animals
Biochemistry and metabolism
Biological and medical sciences
Brain - drug effects
Brain - growth & development
Brain - metabolism
Central nervous system
Development
Fundamental and applied biological sciences. Psychology
Glutamate transporters
Glutamate uptake
Glutamates - pharmacokinetics
Guanosine
Guanosine - pharmacology
In Vitro Techniques
Male
Rats
Rats, Wistar
Vertebrates: nervous system and sense organs
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Summary:The excitotoxicity of the neurotransmitter glutamate has been shown to be connected with many acute and chronic diseases of the CNS. High affinity sodium-dependent glutamate transporters play a key role in maintaining adequate levels of extracellular glutamate. In the present study, we used slices of striatum, hippocampus and cortex from rat brain to describe the in vitro profile of glutamate uptake during development and ageing, and its sensitivity to guanosine. In all structures, glutamate uptake was higher in immature animals. There was a maximum decrease in glutamate uptake in striatum and hippocampus in 15-month-old rats, which later increased, while in cortex there was a significant decrease in rats aged 60 days old. The effect of guanosine seems to be age and structure dependent since the increase in basal glutamate uptake was only seen in slices of cortex from 10-day-old animals.
ISSN:0047-6374
1872-6216
DOI:10.1016/j.mad.2004.04.005