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Postnatal Catch-up Growth After Fetal Protein Restriction Programs Proliferation of Rat Preadipocytes

We studied the in vitro proliferation and differentiation of rat preadipocytes to investigate whether catch‐up growth after prenatal protein restriction may program adipose precursor cells leading to development of increased adipose tissue mass. Pregnant rat dams were fed either an isocaloric low‐pr...

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Published in:Obesity (Silver Spring, Md.) Md.), 2008-12, Vol.16 (12), p.2760-2763
Main Authors: Bol, Vanesa V, Reusens, Brigitte M, Remacle, Claude A
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description We studied the in vitro proliferation and differentiation of rat preadipocytes to investigate whether catch‐up growth after prenatal protein restriction may program adipose precursor cells leading to development of increased adipose tissue mass. Pregnant rat dams were fed either an isocaloric low‐protein diet (LP‐8%) or control diet (C‐20%). During lactation, in order to induce catch‐up growth, dams from LP group were fed with the C diet and litter size was reduced to four pups instead of eight. Preadipocytes were isolated from weanling male pups (28 days of age). Differentiation and proliferation were assessed across time. At late stages of preadipocyte differentiation, no difference was observed in lipid accumulation of C or LP cultures but the mRNA expression of leptin was enhanced in LP cells. At early stages of culture, a higher DNA and protein content accompanied by a higher rate of proliferation was measured in adipocytes from LP cultures. Moreover, the mRNA expression of cyclin D1 was increased in these cells whereas the expression of peroxisome proliferators‐activated receptor γ (PPARγ) and steroyl regulatory element binding protein (SREBP‐1c) was significantly reduced during early stages. The results suggest that prenatal exposure to a LP followed by rapid catch‐up growth is associated with a higher rate for proliferation in preadipocytes.
doi_str_mv 10.1038/oby.2008.417
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source Wiley-Blackwell Read & Publish Collection
subjects Adipocytes
Adipocytes - cytology
Adipocytes - metabolism
Adipose Tissue - growth & development
animal models
Animals
Animals, Newborn
Body fat
Breastfeeding & lactation
Cell culture
Cell Differentiation
Cell Proliferation
compensatory growth
Cyclin D1 - metabolism
dams (mothers)
Diet
Diet, Protein-Restricted
Female
fetus
Growth
Laboratories
Leptin - metabolism
Lipids
low protein diet
Male
Malnutrition
maternal nutrition
postnatal development
PPAR gamma - metabolism
preadipocytes
Pregnancy
protein deficiencies
Proteins
Proteins - analysis
Rats
RNA, Messenger - metabolism
Sterol Regulatory Element Binding Protein 1 - metabolism
suckling
title Postnatal Catch-up Growth After Fetal Protein Restriction Programs Proliferation of Rat Preadipocytes
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