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A paucity of structural integrity in cloned porcine blastocysts produced in vitro
The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE...
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| Published in: | Theriogenology 2004-09, Vol.62 (5), p.779-789 |
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| cites | cdi_FETCH-LOGICAL-c472t-9ed41b71f92c675e8edcefb8cf148beb5cb06a563f6834d3c2facb2b0c9e82843 |
| container_end_page | 789 |
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| container_title | Theriogenology |
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| creator | Koo, Deog-Bon Kang, Yong-Kook Park, Jung Sun Park, Jin-Ki Chang, Won-Kyong Lee, Kyung-Kwang Han, Yong-Mahn |
| description | The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE cells was examined and compared with IVF- and in vivo-derived embryos. NT-derived embryos had a lower developmental competence to the blastocyst stage than IVF-derived embryos (
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| doi_str_mv | 10.1016/j.theriogenology.2003.12.027 |
| format | article |
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P<0.05). Total cell number of NT-derived blastocysts was inferior to that of IVF-derived embryos (
P<0.05), although no difference was detected between the two groups in the ratio of ICM to total cells. However, in vivo-derived blastocysts had a higher proportion of ICM to total cells compared with in vitro-produced embryos (
P<0.01). To investigate what proportions of in vitro-produced porcine embryos represent normal structural integrity, differentially-stained blastocysts were individually classified into three presumptive groups (I: <20%; II: 20–40%; III: >40%) according to the ratio of ICM to total cells. Low proportions of NT- (12.5%, 7/56) and IVF-derived blastocysts (15.8%, 9/57) were assigned to Group II, presumptively having a normal range of structural integrity, whereas, almost all in vivo-derived embryos (97.5%, 39/40) were allocated to Group II. In conclusion, limited structural integrity may lead to the poor survival to term of NT- or IVF-derived porcine embryos produced in vitro.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2003.12.027</identifier><identifier>PMID: 15251229</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blastocyst ; Blastocyst - cytology ; Blastocyst - physiology ; Cell Count ; Cloning, Organism ; Differential staining ; embryo culture ; embryogenesis ; embryonic mortality ; embryonic structures ; Female ; Fertilization in Vitro - veterinary ; In vitro fertilization ; inner cell mass ; Male ; Nuclear transfer ; Nuclear Transfer Techniques ; nuclear transplantation ; Porcine ; Swine ; trophectoderm ; trophoblast</subject><ispartof>Theriogenology, 2004-09, Vol.62 (5), p.779-789</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-9ed41b71f92c675e8edcefb8cf148beb5cb06a563f6834d3c2facb2b0c9e82843</citedby><cites>FETCH-LOGICAL-c472t-9ed41b71f92c675e8edcefb8cf148beb5cb06a563f6834d3c2facb2b0c9e82843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15251229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koo, Deog-Bon</creatorcontrib><creatorcontrib>Kang, Yong-Kook</creatorcontrib><creatorcontrib>Park, Jung Sun</creatorcontrib><creatorcontrib>Park, Jin-Ki</creatorcontrib><creatorcontrib>Chang, Won-Kyong</creatorcontrib><creatorcontrib>Lee, Kyung-Kwang</creatorcontrib><creatorcontrib>Han, Yong-Mahn</creatorcontrib><title>A paucity of structural integrity in cloned porcine blastocysts produced in vitro</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE cells was examined and compared with IVF- and in vivo-derived embryos. NT-derived embryos had a lower developmental competence to the blastocyst stage than IVF-derived embryos (
P<0.05). Total cell number of NT-derived blastocysts was inferior to that of IVF-derived embryos (
P<0.05), although no difference was detected between the two groups in the ratio of ICM to total cells. However, in vivo-derived blastocysts had a higher proportion of ICM to total cells compared with in vitro-produced embryos (
P<0.01). To investigate what proportions of in vitro-produced porcine embryos represent normal structural integrity, differentially-stained blastocysts were individually classified into three presumptive groups (I: <20%; II: 20–40%; III: >40%) according to the ratio of ICM to total cells. Low proportions of NT- (12.5%, 7/56) and IVF-derived blastocysts (15.8%, 9/57) were assigned to Group II, presumptively having a normal range of structural integrity, whereas, almost all in vivo-derived embryos (97.5%, 39/40) were allocated to Group II. In conclusion, limited structural integrity may lead to the poor survival to term of NT- or IVF-derived porcine embryos produced in vitro.</description><subject>Animals</subject><subject>Blastocyst</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - physiology</subject><subject>Cell Count</subject><subject>Cloning, Organism</subject><subject>Differential staining</subject><subject>embryo culture</subject><subject>embryogenesis</subject><subject>embryonic mortality</subject><subject>embryonic structures</subject><subject>Female</subject><subject>Fertilization in Vitro - veterinary</subject><subject>In vitro fertilization</subject><subject>inner cell mass</subject><subject>Male</subject><subject>Nuclear transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>nuclear transplantation</subject><subject>Porcine</subject><subject>Swine</subject><subject>trophectoderm</subject><subject>trophoblast</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqNkFGL1DAQx4Mo3nr6FbQP4ltrJmnTFnw5Dk-FAxE98C0k08mapdusSXqw394suyC--TQw85uZPz_G3gJvgIN6v2vyL4o-bGkJc9geG8G5bEA0XPRP2AaGfqylkPCUbTgfZa1G-HnFXqS04wVUCp6zK-hEB0KMG_btpjqYFX0-VsFVKccV8xrNXPkl0zae-n6pcA4LTdUhRPQLVXY2KQc8ppyqQwzTimVYsEefY3jJnjkzJ3p1qdfs4e7jj9vP9f3XT19ub-5rbHuR65GmFmwPbhSo-o4GmpCcHdBBO1iyHVquTKekU4NsJ4nCGbTCchxpEEMrr9m7892S4PdKKeu9T0jzbBYKa9JK9Vx20BXwwxnEGFKK5PQh-r2JRw1cn5Tqnf5XqT4p1SB0UVrWX1_-rHZP09_li8MCvDkDzgRtirOkH74LDrLob1uQqhB3Z4KKj0dPUSf0tBRrPhJmPQX_f1n-AEIFnH4</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Koo, Deog-Bon</creator><creator>Kang, Yong-Kook</creator><creator>Park, Jung Sun</creator><creator>Park, Jin-Ki</creator><creator>Chang, Won-Kyong</creator><creator>Lee, Kyung-Kwang</creator><creator>Han, Yong-Mahn</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>A paucity of structural integrity in cloned porcine blastocysts produced in vitro</title><author>Koo, Deog-Bon ; Kang, Yong-Kook ; Park, Jung Sun ; Park, Jin-Ki ; Chang, Won-Kyong ; Lee, Kyung-Kwang ; Han, Yong-Mahn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-9ed41b71f92c675e8edcefb8cf148beb5cb06a563f6834d3c2facb2b0c9e82843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Blastocyst</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - physiology</topic><topic>Cell Count</topic><topic>Cloning, Organism</topic><topic>Differential staining</topic><topic>embryo culture</topic><topic>embryogenesis</topic><topic>embryonic mortality</topic><topic>embryonic structures</topic><topic>Female</topic><topic>Fertilization in Vitro - veterinary</topic><topic>In vitro fertilization</topic><topic>inner cell mass</topic><topic>Male</topic><topic>Nuclear transfer</topic><topic>Nuclear Transfer Techniques</topic><topic>nuclear transplantation</topic><topic>Porcine</topic><topic>Swine</topic><topic>trophectoderm</topic><topic>trophoblast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koo, Deog-Bon</creatorcontrib><creatorcontrib>Kang, Yong-Kook</creatorcontrib><creatorcontrib>Park, Jung Sun</creatorcontrib><creatorcontrib>Park, Jin-Ki</creatorcontrib><creatorcontrib>Chang, Won-Kyong</creatorcontrib><creatorcontrib>Lee, Kyung-Kwang</creatorcontrib><creatorcontrib>Han, Yong-Mahn</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koo, Deog-Bon</au><au>Kang, Yong-Kook</au><au>Park, Jung Sun</au><au>Park, Jin-Ki</au><au>Chang, Won-Kyong</au><au>Lee, Kyung-Kwang</au><au>Han, Yong-Mahn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A paucity of structural integrity in cloned porcine blastocysts produced in vitro</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>62</volume><issue>5</issue><spage>779</spage><epage>789</epage><pages>779-789</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE cells was examined and compared with IVF- and in vivo-derived embryos. NT-derived embryos had a lower developmental competence to the blastocyst stage than IVF-derived embryos (
P<0.05). Total cell number of NT-derived blastocysts was inferior to that of IVF-derived embryos (
P<0.05), although no difference was detected between the two groups in the ratio of ICM to total cells. However, in vivo-derived blastocysts had a higher proportion of ICM to total cells compared with in vitro-produced embryos (
P<0.01). To investigate what proportions of in vitro-produced porcine embryos represent normal structural integrity, differentially-stained blastocysts were individually classified into three presumptive groups (I: <20%; II: 20–40%; III: >40%) according to the ratio of ICM to total cells. Low proportions of NT- (12.5%, 7/56) and IVF-derived blastocysts (15.8%, 9/57) were assigned to Group II, presumptively having a normal range of structural integrity, whereas, almost all in vivo-derived embryos (97.5%, 39/40) were allocated to Group II. In conclusion, limited structural integrity may lead to the poor survival to term of NT- or IVF-derived porcine embryos produced in vitro.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15251229</pmid><doi>10.1016/j.theriogenology.2003.12.027</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Blastocyst Blastocyst - cytology Blastocyst - physiology Cell Count Cloning, Organism Differential staining embryo culture embryogenesis embryonic mortality embryonic structures Female Fertilization in Vitro - veterinary In vitro fertilization inner cell mass Male Nuclear transfer Nuclear Transfer Techniques nuclear transplantation Porcine Swine trophectoderm trophoblast |
| title | A paucity of structural integrity in cloned porcine blastocysts produced in vitro |
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