Analysis of benzyldimethyldodecylammonium bromide in chemical disinfectants by liquid chromatography and capillary electrophoresis

• Published in: Journal of Chromatography A 2004-06, Vol.1039 (1), p.209-213
• Main Authors: Ding, Xiaojing, Mou, Shifen, Zhao, Shan
• Format: Article
• Language: English
• Subjects: Analysis; Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Benzalkonium bromide; Benzalkonium Compounds - analysis; Benzyldimethyldodecylammonium bromide; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Disinfectants; Disinfectants - chemistry; Electrophoresis, Capillary - methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General pharmacology; Medical sciences; Pharmacology. Drug treatments; Quaternary ammonium compounds; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet - methods; Surfactants
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.03.076

Two novel analytical methodologies using capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) were developed and compared for the determination of benzyldimethyldodecylammonium bromide (BAB) in commercial compound chemical disinfectants. The LC analysis was performed with a Kromasil C 18 ( 200 mm×4.6 mm, 5 μm) column and a mobile phase of A:B=80:20 (A: acetonitrile, B: 4 mmol/L octanesulfonic sodium—0.02 mol/L acetic sodium, adjusted with acetic acid to pH 5.2) at a flow rate of 1.0 mL/min. Detection was by ultraviolet absorption at 262 nm. The CE analysis was performed in a bare fused-silica capillary with 75 μm i.d. and total length of 46.4 cm with a buffer solution of 50% acetonitrile −50 mmol/L NaH 2PO 4, pH 2.24. The applied voltage was 20 kV. Detection was by ultraviolet absorption at 214 nm. Under optimized conditions, the HPLC retention time and CE migration time for BAB was 9.18 and 5.08 min, respectively. Calibration curves of peak area versus concentration gave correlation coefficients of 0.9996 for HPLC and 0.9994 for CE. The detection limits for HPLC and CE were 1.6 mg/L and 0.2 mg/L, respectively. Average recoveries at three concentration levels (50, 100, 200 mg/L for HPLC; 20, 40, 100 mg/L for CE) were 99.94±1.5, 99.64±1.3 and 99.61±0.4% for HPLC and 120.47±2.6, 102.06±8.7 and 103.05±3.0% for CE, respectively. Although both methods were shown to be suitable for the determination of BAB in commercial disinfectant compounds, CE provided analysis with less solvent purchase/disposal and better column efficiency, whereas HPLC provided superior precision.