Cuticular lipids and desiccation resistance in overwintering larvae of the goldenrod gall fly, Eurosta solidaginis (Diptera: Tephritidae)

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2004-07, Vol.138 (3), p.313-320
• Main Authors: Nelson, Dennis R, Lee, Richard E
• Format: Article
• Language: English
• Subjects: 2-Methylalkanes; Acclimatization - physiology; Animals; Body Water - metabolism; Cold Temperature; dehydration (animal physiology); Desiccation resistance; Diptera; Eurosta solidaginis; Hydrocarbons; Hydrocarbons - metabolism; insect physiology; Iso-alkanes; Larva - physiology; larvae; Lipid Metabolism; lipids; Lipids - chemistry; Mass spectrometry; Methyl-branched alkanes; Overwintering; Seasons; Surface lipids; Tephritidae; Tephritidae - physiology; Water Loss, Insensible - physiology
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpc.2004.04.013

Within their gall, larvae of the goldenrod gall fly ( Eurosta solidaginis) experience severe desiccating conditions as well as highly variable thermal conditions and extreme cold during winter. Through the autumn and early winter, field-collected larvae acquired markedly enhanced resistance to desiccation and freezing. At the same time, they increased their cuticular surface hydrocarbons. Hydrocarbons were the major lipid class extracted by hexane or chloroform from the cuticular surface of overwintering gall fly larvae. The major hydrocarbon classes were the 2-methylalkanes which consisted mainly of 2-methyltriacontane. 2-Methyltriacontane comprised 48–68% of the total hydrocarbons during the larval stages. Total hydrocarbons increased from 122 ng/larva in early third instar larvae collected in September to 4900 ng/larva in those collected in January. Although washing of the cuticular surface with chloroform or chloroform:methanol (2:1, v:v) caused marked increases in rates of water loss, treatment with hexane and methanol had little effect on water loss rates.