Comparative Analysis of Reproduction of Influenza Virus Strains in Cell Lines Perspective for the Creation of Cultural Vaccines Grown on Nutrient Medium on the Basis of Rise Flour Protein Hydrolysate

We studied vaccine strains of influenza viruses A and B during their culturing in MDCK and Vero cells grown in Eagle's MEM medium and in a medium on the basis of enzyme hydrolysate of rise flour proteins with reduced (2%) content of fetal calf serum. Optimal conditions for cell culturing and re...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2008-10, Vol.146 (4), p.547-550
Main Authors: Mazurkova, N. A, Troshkova, G. P, Sumkina, T. P, Kolokol'tsova, T. D, Skarnovich, M. O, Kabanov, A. S, Shishkina, L. N, Sergeev, A. N
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cats
Cell Biology
Cell Line
Cercopithecus aethiops
cultured cells
enzyme hydrolysate
Influenza A virus - growth & development
Influenza A virus - metabolism
Influenza B virus - growth & development
Influenza B virus - metabolism
Influenza Vaccines
Influenza virus
Internal Medicine
Laboratory Medicine
nutrient media
Pathology
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
Vero Cells
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Summary:We studied vaccine strains of influenza viruses A and B during their culturing in MDCK and Vero cells grown in Eagle's MEM medium and in a medium on the basis of enzyme hydrolysate of rise flour proteins with reduced (2%) content of fetal calf serum. Optimal conditions for cell culturing and reproduction of influenza virus strains in these cells were studied. Culturing of vaccine strains of influenza viruses in MDCK and Vero cells grown in nutrient media on the basis of rise flour protein hydrolysate yielded high infection titers, which suggests that this medium can be used for the development of cultural influenza vaccine.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-009-0317-5