Search for Protein Adhesin Gene in Bifidobacterium Longum Genome Using Surface Phage Display Technology

A fragment of the nucleotide sequence encoding polypeptide binding to HT-29 epithelial cells was cloned from VMKB44 Bifidobacterium longum genome library using surface phage display technology. Sequencing of this polypeptide consisting of 26 amino acid residues showed that it is an extracellular fra...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2008-12, Vol.146 (6), p.782-785
Main Authors: Shkoporov, A. N, Khokhlova, E. V, Kafarskaia, L. I, Pavlov, K. A, Smeianov, V. V, Steele, J. L, Efimov, B. A
Format: Article
Language:English
Subjects:
adhesin
Adhesins, Bacterial - chemistry
Adhesins, Bacterial - genetics
adhesion
Amino Acid Sequence
bifidobacteria
Bifidobacterium - genetics
Bifidobacterium longum
Biomedical and Life Sciences
Biomedicine
Cell Biology
Genetics
Genome, Bacterial - genetics
HT-29
HT29 Cells
Humans
Internal Medicine
Laboratory Medicine
Pathology
Peptide Library
phage display
Sequence Alignment
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Summary:A fragment of the nucleotide sequence encoding polypeptide binding to HT-29 epithelial cells was cloned from VMKB44 Bifidobacterium longum genome library using surface phage display technology. Sequencing of this polypeptide consisting of 26 amino acid residues showed that it is an extracellular fragment of a large BL0155 transmembrane protein belonging to the ABC transport protein superfamily. The genes encoding homologues of this protein were detected in genomes of not only bifidobacteria of different species, but also in many other enteric commencals and pathogens.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-009-0423-4