Detection and some properties of cowpea mild mottle virus isolated from soybean in Iran

During 2006-2007 growing seasons, survey were carried to identify a virus disease causing mosaic of soybean in the field in Southern region (Khozestan Province) of Iran. To detect the viral infection, diseased leaf samples showing mild mosaic and leaf malformation were collected from soybean fields...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 2008-12, Vol.11 (23), p.2624-2628
Main Authors: Tavassoli, M, Shahraeen, N, Ghorbani, S
Format: Article
Language:English
Subjects:
Carlavirus - classification
Carlavirus - isolation & purification
Carlavirus - pathogenicity
Carlavirus - ultrastructure
Cowpea mild mottle virus
Enzyme-Linked Immunosorbent Assay
Geography
Glycine max - virology
Iran
Plant Diseases - virology
Plant Leaves - virology
Seasons
Serotyping
Specimen Handling
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Summary:During 2006-2007 growing seasons, survey were carried to identify a virus disease causing mosaic of soybean in the field in Southern region (Khozestan Province) of Iran. To detect the viral infection, diseased leaf samples showing mild mosaic and leaf malformation were collected from soybean fields in Dezful, located in Khozestan Province. Infected samples were carried to the lab in a proper condition on ice packages. TPIA and DAS-ELISA serological tests were applied to identify the viral agent. To investigate the host-range, several indicator plants were mechanically inoculated under green-house condition. Seed transmission of CPMMV was examined using the seeds obtained from infected plants. The virus isolate was not found to be seed-borne in Clark variety of soybean. Different steps of ultracentrifugation including sucrose density gradient (10-40%) were carried out in order to obtain partial purified virus. On the basis of biological, serological and EM results, CPMMV-Carla virus was identified in the infected soybean samples. This is the first report of CPMMV infection of soybean in Iran.
ISSN:1028-8880
DOI:10.3923/pjbs.2008.2624.2628