Medium optimization for the production of cyclic adenosine 3′,5′-monophosphate by Microbacterium sp. no. 205 using response surface methodology

Response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3′,5′-monophosphate (cAMP) with Microbacterium sp. no. 205. A fractional factorial design (211−7) was applied to evaluate the effects of different components in the medium. K2HPO4, MgSO4 a...

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Bibliographic Details
Published in:Bioresource technology 2009-01, Vol.100 (2), p.919-924
Main Authors: Chen, Xiao-Chun, Bai, Jian-Xin, Cao, Jia-Ming, Li, Zhen-Jiang, Xiong, Jian, Zhang, Lei, Hong, Yuan, Ying, Han-Jie
Format: Article
Language:English
Subjects:
Actinomycetales - classification
Actinomycetales - metabolism
Algorithms
Biological and medical sciences
Bioreactors - microbiology
Biotechnology
Cell Culture Techniques - methods
Combinatorial Chemistry Techniques - methods
Computer Simulation
Culture Media - chemistry
Culture Media - metabolism
Cyclic adenosine 3′,5′-monophosphate
Cyclic AMP - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Medium
Methods. Procedures. Technologies
Microbacterium
Microbial engineering. Fermentation and microbial culture technology
Models, Biological
Optimization
Response surface methodology
Species Specificity
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Summary:Response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3′,5′-monophosphate (cAMP) with Microbacterium sp. no. 205. A fractional factorial design (211−7) was applied to evaluate the effects of different components in the medium. K2HPO4, MgSO4 and NaF were found to significantly influence on the cAMP production. The steepest ascent method was used to access the optimal region of the medium composition. The concentrations of the three factors were optimized subsequently using central composite design and response surface methodology. The optimal medium composition to achieve the optimal cAMP production was determined (g/L): K2HPO4, 12.78; MgSO4, 3.53 and NaF, 0.18. The corresponding cAMP concentration was 8.50g/L, which was about 1.8-fold increase compared with that using the original medium. Validation experiments were also carried out to prove the adequacy and the accuracy of the model obtained. The cAMP fermentation in 5L fermenter reached 9.87g/L.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2008.07.062