Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: Application for textile dye degradation

Non-porous poly(glycidyl methacrylate/ethyleneglycol dimetacrylate) (poly(GMA/EGDMA)) beads were prepared by suspension polymerization. The enzyme (i.e. laccase) was covalently immobilized onto plain and spacer-arm attached poly(GMA/EGDMA) beads. The amount of immobilized enzyme on the plain and spa...

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Bibliographic Details
Published in:Bioresource technology 2009-01, Vol.100 (2), p.665-669
Main Authors: YAKUP ARICA, M, ALTMTAS, Begum, BAYRAMOGLU, Gülay
Format: Article
Language:English
Subjects:
Adsorption
Biological and medical sciences
Biotechnology
Coloring Agents - chemistry
Coloring Agents - isolation & purification
Dye removal
Environment and pollution
Enzyme Activation
Enzyme reactor
Enzyme technology
Enzymes, Immobilized - chemistry
Epoxy Compounds - chemistry
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Immobilized enzyme
Industrial applications and implications. Economical aspects
Laccase
Laccase - chemistry
Methacrylates - chemistry
Methods. Procedures. Technologies
Microspheres
Porosity
Textiles
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Summary:Non-porous poly(glycidyl methacrylate/ethyleneglycol dimetacrylate) (poly(GMA/EGDMA)) beads were prepared by suspension polymerization. The enzyme (i.e. laccase) was covalently immobilized onto plain and spacer-arm attached poly(GMA/EGDMA) beads. The amount of immobilized enzyme on the plain and spacer-arm attached beads was determined as 5.6 and 4.9mg/g, respectively. The maximum activity (Vmax) and Michaelis constant (Km) of laccase immobilized on the spacer-arm attached beads, were found to be 77.6U/min and 0.47mM, respectively. Finally, the immobilized laccase was operated in a batch system, and textile dye Reactive Red 120 was successfully decolorized in the enzyme reactor.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2008.07.038