Fluorescence In Situ Hybridization Analysis Reveals Multiple Loci of Knob-associated DNA Elements in One-knob and Knobless Maize Lines

Fluorescence in situ hybridization analyses were conducted to examine the presence or absence of the 180- and 350-bp knob-associated tandem repeats in maize strains previously defined as “one-knob” or “knobless.” Multiple loci were found to hybridize to these two repeats in all maize lines analyzed....

Full description

Saved in:
Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 2004-08, Vol.52 (8), p.1113-1116
Main Authors: Adawy, Sami S.M, Stupar, Robert M, Jiang, Jiming
Format: Article
Language:English
Subjects:
DNA, Plant - genetics
Heterochromatin - genetics
In Situ Hybridization
Tandem Repeat Sequences
Zea mays - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c276t-be3fdeea9401734399f4498cb3b63146b792681968a50a52188c4db48bb851873
cites cdi_FETCH-LOGICAL-c276t-be3fdeea9401734399f4498cb3b63146b792681968a50a52188c4db48bb851873
container_end_page 1116
container_issue 8
container_start_page 1113
container_title The journal of histochemistry and cytochemistry
container_volume 52
creator Adawy, Sami S.M
Stupar, Robert M
Jiang, Jiming
description Fluorescence in situ hybridization analyses were conducted to examine the presence or absence of the 180- and 350-bp knob-associated tandem repeats in maize strains previously defined as “one-knob” or “knobless.” Multiple loci were found to hybridize to these two repeats in all maize lines analyzed. Our results show that the number of 180- and 350-bp repeat loci do not correlate with the number of knobs in maize and that these tandem repeats are not independently sufficient to confer knob heterochromatin, even when present at megabase sizes.
doi_str_mv 10.1369/jhc.4B6335.2004
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66718658</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1369_jhc.4B6335.2004</sage_id><sourcerecordid>66718658</sourcerecordid><originalsourceid>FETCH-LOGICAL-c276t-be3fdeea9401734399f4498cb3b63146b792681968a50a52188c4db48bb851873</originalsourceid><addsrcrecordid>eNp9kLtu2zAUQImiQeOmnbsVnDpVDik-RI1umhfiNkAfM0FKVzFdinJIqYbzAfnuMrWBbJkIEuce8B6EPlAyp0zWp-tVM-dfJGNiXhLCX6EZFYIWgnD-Gs0IKcsiP_Bj9DalNSGUc6HeoGMqSqGoUjP0eOGnIUJqIDSArwP-6cYJX-1sdK17MKMbAl4E43fJJfwD_oLxCX-b_Og2HvByaBweOnwTBluYlPLVjNDir98X-NxDD2FM2AV8G6D4kxlsQvsf9pCyxriH7HAB0jt01GUzvD-cJ-j3xfmvs6tieXt5fbZYFk1ZybGwwLoWwNSc0IpxVtcd57VqLLOSUS5tVZdS0VoqI4gRZV6x4a3lylolqKrYCfq0927icD9BGnXv8u7emwDDlLSUFVVSqAye7sEmDilF6PQmut7EnaZEP6XXOb3ep9dP6fPEx4N6sj20z_yhdQY-74Fk7kCvhynmrukF3-GrK3e32roIOvXG-2ynervdilIrTSll7B-4yZqh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66718658</pqid></control><display><type>article</type><title>Fluorescence In Situ Hybridization Analysis Reveals Multiple Loci of Knob-associated DNA Elements in One-knob and Knobless Maize Lines</title><source>Sage Journals Online</source><source>Free Full-Text Journals in Chemistry</source><creator>Adawy, Sami S.M ; Stupar, Robert M ; Jiang, Jiming</creator><creatorcontrib>Adawy, Sami S.M ; Stupar, Robert M ; Jiang, Jiming</creatorcontrib><description>Fluorescence in situ hybridization analyses were conducted to examine the presence or absence of the 180- and 350-bp knob-associated tandem repeats in maize strains previously defined as “one-knob” or “knobless.” Multiple loci were found to hybridize to these two repeats in all maize lines analyzed. Our results show that the number of 180- and 350-bp repeat loci do not correlate with the number of knobs in maize and that these tandem repeats are not independently sufficient to confer knob heterochromatin, even when present at megabase sizes.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1369/jhc.4B6335.2004</identifier><identifier>PMID: 15258188</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>DNA, Plant - genetics ; Heterochromatin - genetics ; In Situ Hybridization ; Tandem Repeat Sequences ; Zea mays - genetics</subject><ispartof>The journal of histochemistry and cytochemistry, 2004-08, Vol.52 (8), p.1113-1116</ispartof><rights>2004 Authors</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c276t-be3fdeea9401734399f4498cb3b63146b792681968a50a52188c4db48bb851873</citedby><cites>FETCH-LOGICAL-c276t-be3fdeea9401734399f4498cb3b63146b792681968a50a52188c4db48bb851873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925,79364</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15258188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adawy, Sami S.M</creatorcontrib><creatorcontrib>Stupar, Robert M</creatorcontrib><creatorcontrib>Jiang, Jiming</creatorcontrib><title>Fluorescence In Situ Hybridization Analysis Reveals Multiple Loci of Knob-associated DNA Elements in One-knob and Knobless Maize Lines</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>Fluorescence in situ hybridization analyses were conducted to examine the presence or absence of the 180- and 350-bp knob-associated tandem repeats in maize strains previously defined as “one-knob” or “knobless.” Multiple loci were found to hybridize to these two repeats in all maize lines analyzed. Our results show that the number of 180- and 350-bp repeat loci do not correlate with the number of knobs in maize and that these tandem repeats are not independently sufficient to confer knob heterochromatin, even when present at megabase sizes.</description><subject>DNA, Plant - genetics</subject><subject>Heterochromatin - genetics</subject><subject>In Situ Hybridization</subject><subject>Tandem Repeat Sequences</subject><subject>Zea mays - genetics</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kLtu2zAUQImiQeOmnbsVnDpVDik-RI1umhfiNkAfM0FKVzFdinJIqYbzAfnuMrWBbJkIEuce8B6EPlAyp0zWp-tVM-dfJGNiXhLCX6EZFYIWgnD-Gs0IKcsiP_Bj9DalNSGUc6HeoGMqSqGoUjP0eOGnIUJqIDSArwP-6cYJX-1sdK17MKMbAl4E43fJJfwD_oLxCX-b_Og2HvByaBweOnwTBluYlPLVjNDir98X-NxDD2FM2AV8G6D4kxlsQvsf9pCyxriH7HAB0jt01GUzvD-cJ-j3xfmvs6tieXt5fbZYFk1ZybGwwLoWwNSc0IpxVtcd57VqLLOSUS5tVZdS0VoqI4gRZV6x4a3lylolqKrYCfq0927icD9BGnXv8u7emwDDlLSUFVVSqAye7sEmDilF6PQmut7EnaZEP6XXOb3ep9dP6fPEx4N6sj20z_yhdQY-74Fk7kCvhynmrukF3-GrK3e32roIOvXG-2ynervdilIrTSll7B-4yZqh</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Adawy, Sami S.M</creator><creator>Stupar, Robert M</creator><creator>Jiang, Jiming</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Fluorescence In Situ Hybridization Analysis Reveals Multiple Loci of Knob-associated DNA Elements in One-knob and Knobless Maize Lines</title><author>Adawy, Sami S.M ; Stupar, Robert M ; Jiang, Jiming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c276t-be3fdeea9401734399f4498cb3b63146b792681968a50a52188c4db48bb851873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>DNA, Plant - genetics</topic><topic>Heterochromatin - genetics</topic><topic>In Situ Hybridization</topic><topic>Tandem Repeat Sequences</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adawy, Sami S.M</creatorcontrib><creatorcontrib>Stupar, Robert M</creatorcontrib><creatorcontrib>Jiang, Jiming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adawy, Sami S.M</au><au>Stupar, Robert M</au><au>Jiang, Jiming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence In Situ Hybridization Analysis Reveals Multiple Loci of Knob-associated DNA Elements in One-knob and Knobless Maize Lines</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>52</volume><issue>8</issue><spage>1113</spage><epage>1116</epage><pages>1113-1116</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>Fluorescence in situ hybridization analyses were conducted to examine the presence or absence of the 180- and 350-bp knob-associated tandem repeats in maize strains previously defined as “one-knob” or “knobless.” Multiple loci were found to hybridize to these two repeats in all maize lines analyzed. Our results show that the number of 180- and 350-bp repeat loci do not correlate with the number of knobs in maize and that these tandem repeats are not independently sufficient to confer knob heterochromatin, even when present at megabase sizes.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>15258188</pmid><doi>10.1369/jhc.4B6335.2004</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-1554
ispartof The journal of histochemistry and cytochemistry, 2004-08, Vol.52 (8), p.1113-1116
issn 0022-1554
1551-5044
language eng
recordid cdi_proquest_miscellaneous_66718658
source Sage Journals Online; Free Full-Text Journals in Chemistry
subjects DNA, Plant - genetics
Heterochromatin - genetics
In Situ Hybridization
Tandem Repeat Sequences
Zea mays - genetics
title Fluorescence In Situ Hybridization Analysis Reveals Multiple Loci of Knob-associated DNA Elements in One-knob and Knobless Maize Lines
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T20%3A43%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fluorescence%20In%20Situ%20Hybridization%20Analysis%20Reveals%20Multiple%20Loci%20of%20Knob-associated%20DNA%20Elements%20in%20One-knob%20and%20Knobless%20Maize%20Lines&rft.jtitle=The%20journal%20of%20histochemistry%20and%20cytochemistry&rft.au=Adawy,%20Sami%20S.M&rft.date=2004-08-01&rft.volume=52&rft.issue=8&rft.spage=1113&rft.epage=1116&rft.pages=1113-1116&rft.issn=0022-1554&rft.eissn=1551-5044&rft_id=info:doi/10.1369/jhc.4B6335.2004&rft_dat=%3Cproquest_cross%3E66718658%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c276t-be3fdeea9401734399f4498cb3b63146b792681968a50a52188c4db48bb851873%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=66718658&rft_id=info:pmid/15258188&rft_sage_id=10.1369_jhc.4B6335.2004&rfr_iscdi=true