Molecular detection of norovirus in sheep and pigs in New Zealand farms

• Published in: Veterinary microbiology 2009, Vol.133 (1), p.184-189
• Main Authors: Wolf, Sandro, Williamson, Wendy, Hewitt, Joanne, Lin, Susan, Rivera-Aban, Malet, Ball, Andrew, Scholes, Paula, Savill, Marion, Greening, Gail E.
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Biological and medical sciences; Caliciviridae Infections - transmission; Caliciviridae Infections - veterinary; Caliciviridae Infections - virology; diagnostic techniques; disease detection; disease prevalence; disease surveillance; epidemiological studies; feces; Feces - virology; Fundamental and applied biological sciences. Psychology; gastroenteritis; Gastroenteritis - veterinary; Gastroenteritis - virology; Genotype; Humans; microbial genetics; Microbiology; Miscellaneous; molecular epidemiology; molecular genetics; Molecular Sequence Data; New Zealand; Norovirus; Norovirus - classification; Norovirus - genetics; Norovirus - isolation & purification; nucleotide sequences; pathogen identification; Phylogeny; Pigs; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse Transcriptase Polymerase Chain Reaction - veterinary; risk assessment; RNA, Viral - chemistry; RNA, Viral - genetics; Sheep; sheep diseases; Sheep Diseases - transmission; Sheep Diseases - virology; Species Specificity; Swine; swine diseases; Swine Diseases - transmission; Swine Diseases - virology; validity; viral diseases of animals and humans; Virology; Zoonoses - transmission; Zoonoses - virology
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2008.06.019

Human norovirus (NoV) is reportedly the major cause of non-bacterial gastroenteritis outbreaks worldwide and is commonly associated with water- and food-borne transmission via the faecal–oral route. Aside from humans, norovirus has been detected in pigs, cattle and mice. The close relatedness of some human and animal noroviruses has raised concerns about potential zoonotic transmission. Our laboratory recently reported the development of a multiplex real-time RT-PCR for the detection and genotyping of norovirus of genogroups I–III. Here we report a study of 56 faecal specimens from pigs and sheep that were collected and screened for noroviruses using this assay. Norovirus was found in 2/23 (9%) of porcine specimens (all were genogroup II) and in 8/33 (24%) of ovine specimens (all were genogroup III). Samples tested positive for norovirus were verified by conventional RT-PCR with different primer sets. Genomes of representative porcine and ovine norovirus strains underwent partial sequence analysis (343 and 2045 bases, respectively). This is the first report describing norovirus in sheep.