Detection of endogenous boldenone in the entire male horses

Boldenone (1,2-dehydrotestosterone) is a common veterinary anabolic agent. Its structure is very similar to testosterone. Testosterone is endogenous in the horse, whereas there has been no report concerning the detection of endogenous boldenone. This paper reports the direct observation of sulphate...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-09, Vol.808 (2), p.287-294
Main Authors: Ho, Emmie N.M., Yiu, Kenneth C.H., Tang, Francis P.W., Dehennin, Louis, Plou, Philippe, Bonnaire, Yves, Wan, Terence S.M.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Boldenone
Chromatography, Liquid - methods
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Horses
Male
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Testosterone - analogs & derivatives
Testosterone - urine
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Summary:Boldenone (1,2-dehydrotestosterone) is a common veterinary anabolic agent. Its structure is very similar to testosterone. Testosterone is endogenous in the horse, whereas there has been no report concerning the detection of endogenous boldenone. This paper reports the direct observation of sulphate conjugate of boldenone in equine urine from entires. The detection procedures involved solid-phase extraction, immunoaffinity column (IAC) purification, and then LC–MS–MS analysis on a Q-ToF instrument. The identification of boldenone sulphate has provided direct evidence for the endogenous nature of boldenone in entire male horses. Quantification data for the normal level of boldenone in Hong Kong racehorses will also be discussed.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2004.05.018