Cloning and functional analysis of wheat V-H⁺-ATPase subunit genes

The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H⁺-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-...

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Bibliographic Details
Published in:Plant molecular biology 2009, Vol.69 (1-2), p.33-46
Main Authors: Zhao, Qian, Zhao, Yan-Jun, Zhao, Bao-Cun, Ge, Rong-Chao, Li, Ming, Shen, Yin-Zhu, Huang, Zhan-Jing
Format: Article
Language:English
Subjects:
Base Sequence
Biochemistry
Biomedical and Life Sciences
Cloning
Cloning, Molecular
Crop science
DNA Primers
Electrophoresis, Gel, Two-Dimensional
Enzymes
Genes
Genes, Plant
Life Sciences
Plant biology
Plant growth
Plant Pathology
Plant Sciences
Proton-Translocating ATPases - genetics
Reverse Transcriptase Polymerase Chain Reaction
Salt tolerance
Seed germination
Seedlings
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Transgenic plants
Triticum - enzymology
Triticum - genetics
Wheat
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Summary:The root microsomal proteomes of salt-tolerant and salt-sensitive wheat lines under salt stress were analyzed by two-dimensional electrophoresis and mass spectrum. A wheat V-H⁺-ATPase E subunit protein was obtained whose expression was enhanced by salt stress. In silicon cloning identified the full-length cDNA sequences of nine subunits and partial cDNA sequences of two subunits of wheat V-H⁺-ATPase. The expression profiles of these V-H⁺-ATPase subunits in roots and leaves of both salt-tolerant and salt-sensitive wheat lines under salt and abscisic acid (ABA) stress were analyzed. The results indicate that the coordinated enhancement of the expression of V-H⁺-ATPase subunits under salt and ABA stress is an important factor determining improved salt tolerance in wheat. The expression of these subunits was tissue-specific. Overexpression of the E subunit by transgenic Arabidopsis thaliana was able to enhance seed germination, root growth and adult seedling growth under salt stress.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-008-9403-8