Hydrogen photoproduction by nutrient-deprived Chlamydomonas reinhardtii cells immobilized within thin alginate films under aerobic and anaerobic conditions

A new technique for immobilizing H₂-photoproducing green algae within a thin (

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Published in:Biotechnology and bioengineering 2009, Vol.102 (1), p.50-58
Main Authors: Kosourov, Sergey N, Seibert, Michael
Format: Article
Language:English
Subjects:
Acetates - metabolism
Aerobiosis
Algae
alginate
Alginates
Anaerobic conditions
Anaerobiosis
Animals
Antiprotozoal Agents - antagonists & inhibitors
Antiprotozoal Agents - pharmacology
Aquatic plants
Biological and medical sciences
Biopolymers
Biotechnology
Cells
Cells, Immobilized - metabolism
Chlamydomonas reinhardtii
Chlamydomonas reinhardtii - metabolism
Fundamental and applied biological sciences. Psychology
General aspects
Glucuronic Acid
green algae
Hexuronic Acids
Hydrogen
Hydrogen - metabolism
hydrogen photoproduction
hydrogenase
immobilization
Immobilization techniques
Inactivation
Light
Methods. Procedures. Technologies
Oxygen - antagonists & inhibitors
Oxygen - pharmacology
oxygen resistance
Polymers
Sulfur
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cited_by cdi_FETCH-LOGICAL-c5040-b5812e195d08c19f2977e69be21a7c769727617b6899426b20b9e5d038151fbd3
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container_title Biotechnology and bioengineering
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creator Kosourov, Sergey N
Seibert, Michael
description A new technique for immobilizing H₂-photoproducing green algae within a thin (
doi_str_mv 10.1002/bit.22050
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Alginate films with entrapped sulfur/phosphorus-deprived Chlamydomonas reinhardtii, strain cc124, cells demonstrate (a) higher cell density (up to 2,000 μg Chl mL⁻¹ of matrix), (b) kinetics of H₂ photoproduction similar to sulfur-deprived suspension cultures, (c) higher specific rates (up to 12.5 μmol mg⁻¹ Chl h⁻¹) of H₂ evolution, (d) light conversion efficiencies to H₂ of over 1% and (e) unexpectedly high resistance of the H₂-photoproducing system to inactivation by atmospheric O₂. The algal cells, entrapped in alginate and then placed in vials containing 21% O₂ in the headspace, evolved up to 67% of the H₂ gas produced under anaerobic conditions. The results indicate that the lower susceptibility of the immobilized algal H₂-producing system to inactivation by O₂ depends on two factors: (a) the presence of acetate in the medium, which supports higher rates of respiration and (b) the capability of the alginate polymer itself to effectively separate the entrapped cells from O₂ in the liquid and headspace and restrict O₂ diffusion into the matrix. The strategy presented for immobilizing algal cells within thin polymeric matrices shows the potential for scale-up and possible future applications. Biotechnol. Bioeng. 2008. Biotechnol. Bioeng. 2009;102: 50-58.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.22050</identifier><identifier>PMID: 18823051</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Acetates - metabolism ; Aerobiosis ; Algae ; alginate ; Alginates ; Anaerobic conditions ; Anaerobiosis ; Animals ; Antiprotozoal Agents - antagonists &amp; inhibitors ; Antiprotozoal Agents - pharmacology ; Aquatic plants ; Biological and medical sciences ; Biopolymers ; Biotechnology ; Cells ; Cells, Immobilized - metabolism ; Chlamydomonas reinhardtii ; Chlamydomonas reinhardtii - metabolism ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glucuronic Acid ; green algae ; Hexuronic Acids ; Hydrogen ; Hydrogen - metabolism ; hydrogen photoproduction ; hydrogenase ; immobilization ; Immobilization techniques ; Inactivation ; Light ; Methods. Procedures. 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Bioeng</addtitle><description>A new technique for immobilizing H₂-photoproducing green algae within a thin (&lt;400 μm) alginate film has been developed. Alginate films with entrapped sulfur/phosphorus-deprived Chlamydomonas reinhardtii, strain cc124, cells demonstrate (a) higher cell density (up to 2,000 μg Chl mL⁻¹ of matrix), (b) kinetics of H₂ photoproduction similar to sulfur-deprived suspension cultures, (c) higher specific rates (up to 12.5 μmol mg⁻¹ Chl h⁻¹) of H₂ evolution, (d) light conversion efficiencies to H₂ of over 1% and (e) unexpectedly high resistance of the H₂-photoproducing system to inactivation by atmospheric O₂. The algal cells, entrapped in alginate and then placed in vials containing 21% O₂ in the headspace, evolved up to 67% of the H₂ gas produced under anaerobic conditions. The results indicate that the lower susceptibility of the immobilized algal H₂-producing system to inactivation by O₂ depends on two factors: (a) the presence of acetate in the medium, which supports higher rates of respiration and (b) the capability of the alginate polymer itself to effectively separate the entrapped cells from O₂ in the liquid and headspace and restrict O₂ diffusion into the matrix. The strategy presented for immobilizing algal cells within thin polymeric matrices shows the potential for scale-up and possible future applications. Biotechnol. Bioeng. 2008. Biotechnol. Bioeng. 2009;102: 50-58.</description><subject>Acetates - metabolism</subject><subject>Aerobiosis</subject><subject>Algae</subject><subject>alginate</subject><subject>Alginates</subject><subject>Anaerobic conditions</subject><subject>Anaerobiosis</subject><subject>Animals</subject><subject>Antiprotozoal Agents - antagonists &amp; inhibitors</subject><subject>Antiprotozoal Agents - pharmacology</subject><subject>Aquatic plants</subject><subject>Biological and medical sciences</subject><subject>Biopolymers</subject><subject>Biotechnology</subject><subject>Cells</subject><subject>Cells, Immobilized - metabolism</subject><subject>Chlamydomonas reinhardtii</subject><subject>Chlamydomonas reinhardtii - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glucuronic Acid</subject><subject>green algae</subject><subject>Hexuronic Acids</subject><subject>Hydrogen</subject><subject>Hydrogen - metabolism</subject><subject>hydrogen photoproduction</subject><subject>hydrogenase</subject><subject>immobilization</subject><subject>Immobilization techniques</subject><subject>Inactivation</subject><subject>Light</subject><subject>Methods. Procedures. 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subjects Acetates - metabolism
Aerobiosis
Algae
alginate
Alginates
Anaerobic conditions
Anaerobiosis
Animals
Antiprotozoal Agents - antagonists & inhibitors
Antiprotozoal Agents - pharmacology
Aquatic plants
Biological and medical sciences
Biopolymers
Biotechnology
Cells
Cells, Immobilized - metabolism
Chlamydomonas reinhardtii
Chlamydomonas reinhardtii - metabolism
Fundamental and applied biological sciences. Psychology
General aspects
Glucuronic Acid
green algae
Hexuronic Acids
Hydrogen
Hydrogen - metabolism
hydrogen photoproduction
hydrogenase
immobilization
Immobilization techniques
Inactivation
Light
Methods. Procedures. Technologies
Oxygen - antagonists & inhibitors
Oxygen - pharmacology
oxygen resistance
Polymers
Sulfur
title Hydrogen photoproduction by nutrient-deprived Chlamydomonas reinhardtii cells immobilized within thin alginate films under aerobic and anaerobic conditions
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