Changes in polyamine pattern are involved in floral initiation and development in Polianthes tuberosa

• Published in: Journal of plant physiology 2004-06, Vol.161 (6), p.709-713
• Main Authors: Huang, Chiung-Kuei, Chang, Bao-Su, Wang, Kuo-Cheng, Her, Sheng-Jou, Chen, Tian-Wen, Chen, Yun-An, Cho, Chung-Lung, Liao, Li-Jen, Huang, Kuang-Liang, Chen, Wen-Shaw, Liu, Zin-Huang
• Format: Article
• Language: English
• Subjects: arginine decarboxylase; Asparagaceae - enzymology; Asparagaceae - growth & development; Biological and medical sciences; Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation; biosynthesis; cadaverine; Carboxy-Lyases - metabolism; Endogenous polyamines; enzyme activity; flowering; Flowers - physiology; Fundamental and applied biological sciences. Psychology; ornithine decarboxylase; Ornithine Decarboxylase - metabolism; plant physiology; Plant physiology and development; Polianthes tuberosa; Polyamines - metabolism; putrescine; signal transduction; spermidine; spermidine synthase; spermine; Vegetative and sexual reproduction, floral biology, fructification
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1078/0176-1617-01256

In the day-neutral plant Polianthes tuberosa (cv. Double) putrescine and spermine in corms at the early floral initiation stage decreased by 26 and 36 percnt;, respectively, compared with that in the vegetative stage. In contrast, a sharp increase in spermidine and cadaverine titers in corms was recorded at the early floral initiation stage. However, cadaverine in corms disappeared at the flower development stage. Polyamines in the roots were generally lower than those in the leaves and corms. In no case was the change in endogenous polyamine titers in the roots and leaves associated with floral initiation and flower development in P. tuberosa. Exogenous application of spermidine at 5, 25 or 150 μg per plant at the vegetative stage did not affect flower primordium counts. However, addition of a spermidine synthase inhibitor, cyclohexylamine, at 150 or 250 μg per plant (each dose was applied two times in total at an interval of 4 days) significantly reduced flower primordium counts, indicating that spermidine is involved in floral initiation and floral development in P. tuberosa. In P. tuberosa corms at the vegetative stage arginine decarboxylase activity rises and decreases at the early floral initiation stage. In contrast, ornithine decarboxylase activity reaches the highest level at the early floral initiation stage and declines significantly at the vegetative stage. Results indicate that an increase in spermidine and a transient increase in cadaverine titers in the corms seem characteristic of early floral initiation in P. tuberosa. It is also suggested that a significant reduction in putrescine and spermine in the corms is involved in the early floral initiation in P. tuberosa.