Molecular and phylogenetic characterization of Theileria spp. parasites in autochthonous bovines (Mirandesa breed) in Portugal

A survey was conducted during the months of April–June 2003 in the northeast Portugal (Bragança district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 11...

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Bibliographic Details
Published in:Veterinary parasitology 2004-08, Vol.123 (1), p.17-23
Main Authors: Brı́gido, Clarisse, da Fonseca, Isabel Pereira, Parreira, Ricardo, Fazendeiro, Isabel, do Rosário, Virgı́lio E, Centeno-Lima, Sónia
Format: Article
Language:English
Subjects:
Animals
Babesia
Babesia - genetics
Babesia - isolation & purification
babesiosis
Babesiosis - blood
Babesiosis - parasitology
Babesiosis - veterinary
Base Sequence
Bos taurus
Bovine Mirandesa breed
Cattle
cattle breeds
Cattle Diseases - blood
Cattle Diseases - parasitology
disease prevalence
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
epidemiological studies
Molecular Sequence Data
Nucleic Acid Hybridization
nucleotide sequences
pathogen identification
Phylogenetic analysis
Phylogeny
Polymerase Chain Reaction - veterinary
Portugal
ribosomal DNA
RLB
RNA, Ribosomal, 18S - chemistry
RNA, Ribosomal, 18S - genetics
Sequence Alignment
Theileria
Theileria - genetics
Theileria - isolation & purification
Theileria buffeli
Theileria buffeli/orientalis group
Theileriasis - blood
Theileriasis - parasitology
theileriosis
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Summary:A survey was conducted during the months of April–June 2003 in the northeast Portugal (Bragança district) in order to characterize the hemoparasite population of an autochthonous Mirandesa breed of Bos taurus. The polymerase chain reaction (PCR) analysis of the bovine blood revealed that 3 out of 116 animals were infected with Theileria and/or Babesia parasites, while reverse line blot hybridisation (RLB) analysis showed that these animals were infected with Theileria buffeli/ orientalis. Cloning and sequencing confirmed the RLB results. Database sequence searches combined with phylogenetic analysis of the partial 18S ribosomal RNA gene sequences obtained enabled us to place the parasites in question as members of the T. buffeli/ orientalis group, confirming the PCR/RLB diagnosis.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2004.05.024