Use of a PCR-based approach for sequencing whole mitochondrial genomes of insects: two examples (cockroach and dragonfly) based on the method developed for decapod crustaceans

Recent development of a PCR-based approach for sequencing vertebrate mitochondrial genomes has attracted much attention as being more rapid and economical than traditional methods using cloned mtDNA and primer walking. Such a method has not been available for insect mitochondrial genomes, despite wi...

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Bibliographic Details
Published in:Insect molecular biology 2004-08, Vol.13 (4), p.435-442
Main Authors: Yamauchi, M.M, Miya, M.U, Nishida, M
Format: Article
Language:English
Subjects:
Animals
Base Composition
Base Sequence
Blattaria
DNA Primers
DNA, Mitochondrial - genetics
Gene Order
genes
genome
genomics
Insecta
Insecta - genetics
Libellulidae
long PCR
mitochondrial DNA
Molecular Sequence Data
Nucleic Acid Conformation
nucleotide sequences
Orthetrum triangulare melania
Orthetrum triangulare melania (Odonata)
Periplaneta fuliginosa
Periplaneta fuliginosa (Blattaria)
polymerase chain reaction
Polymerase Chain Reaction - methods
RNA, Transfer - genetics
sequence analysis
Sequence Analysis, DNA - methods
versatile PCR primer
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Summary:Recent development of a PCR-based approach for sequencing vertebrate mitochondrial genomes has attracted much attention as being more rapid and economical than traditional methods using cloned mtDNA and primer walking. Such a method has not been available for insect mitochondrial genomes, despite widespread use of them for the molecular phylogenetic, biogeographical and population genetic markers. A recently developed PCR-based approach for sequencing whole mitochondrial genomes of decapod crustaceans, which included the design of many versatile PCR primers for the latter, was applied with the same primers sets to mitochondrial genomes of two insects, smokybrown cockroach Periplaneta fuliginosa (Serville, 1839) and skimmer dragonfly Orthetrum triangulare melania (Selys, 1883). Almost the entire region of the two mitochondrial genomes was successfully sequenced. Features of the two mitochondrial genomes are described and the usefulness of this PCR-based approach for sequencing insect mitochondrial genomes demonstrated.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.0962-1075.2004.00505.x