The complete mitochondrial genome of Macaca thibetana and a novel nuclear mitochondrial pseudogene

The complete mitochondrial genome of Macaca thibetana was determined by the long and accurate polymerase chain reaction (LA-PCR) and primer walking sequencing methods. It is 16,540 bp and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one control region. Most co...

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Bibliographic Details
Published in:Gene 2009-01, Vol.429 (1), p.31-36
Main Authors: Li, Deming, Fan, Longqing, Zeng, Bo, Yin, Hailin, Zou, Fangdong, Wang, Hongxing, Meng, Yang, King, Emily, Yue, Bisong
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - genetics
Cercopithecidae
Codon - genetics
DNA, Mitochondrial - genetics
Female
Genome, Mitochondrial - genetics
Macaca - genetics
Macaca thibetana
Mitochondrial genome
Molecular Sequence Data
Nuclear mitochondrial pseudogene
Open Reading Frames - genetics
Phylogenetic analysis
Phylogeny
Pseudogenes - genetics
RNA, Ribosomal - genetics
RNA, Transfer - genetics
Sequence Analysis, DNA
Time Factors
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Summary:The complete mitochondrial genome of Macaca thibetana was determined by the long and accurate polymerase chain reaction (LA-PCR) and primer walking sequencing methods. It is 16,540 bp and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one control region. Most codon usage followed the typical pattern of vertebrates. Two rare start codons were found, in which GTG initiated the NADH dehydrogenase subunit 1 (ND1) gene and the ATP8 gene, and ATT initiated the ND2 gene. A new mitochondrial DNA-like sequence (2003 bp) in the nuclear genome of M. thibetana was found. It matched with the 3′ end of the ND1 gene, the tRNA Ile-tRNA Gln-tRNA Met genes, the ND2 gene, and the 5′ end of the tRNA Trp gene. Sequence divergence between the nuclear pseudogene and the mitochondrial homologue suggested that the translocation of this mtDNA fragment into the nuclear genome occurred 3.16 ∼ 3.48 million years ago (MYA). Molecular phylogenetic analysis of 16 Cercopithecidae species was performed using sequences from 12 concatenated heavy-strand encoded protein coding genes. The results provided more evidence to support previous morphological and chromosomal studies on Cercopithecidae.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2008.10.010