Differences in nitrate reduction between Mycobacterium tuberculosis and Mycobacterium bovis are due to differential expression of both narGHJI and narK2

Both Mycobacterium tuberculosis and Mycobacterium bovis can produce tuberculosis in humans. Mycobacterium tuberculosis has low nitrate reductase activity during aerobic growth (AG), but shows strong hypoxic induction. Virulent M. bovis has weak activity during AG with no hypoxic induction. Bacille C...

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Bibliographic Details
Published in:FEMS microbiology letters 2009-01, Vol.290 (2), p.129-134
Main Authors: Sohaskey, Charles D, Modesti, Lucia
Format: Article
Language:English
Subjects:
Aerobiosis
Anion Transport Proteins - genetics
Anion Transport Proteins - metabolism
Bacillus Calmette-Guerin vaccine
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
BCG
Biological and medical sciences
bovis
Cattle
Cloning, Molecular
Enzymes
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genetics
Humans
Hypoxia
Metabolism. Enzymes
Microbiology
mycobacteria
Mycobacterium bovis
Mycobacterium bovis - enzymology
Mycobacterium bovis - genetics
Mycobacterium bovis - pathogenicity
Mycobacterium tuberculosis
Mycobacterium tuberculosis - enzymology
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - pathogenicity
Nitrate reductase
Nitrate Reductase - genetics
Nitrate Reductase - metabolism
Nitrate reduction
Nitrates
Nitrates - metabolism
Operon
Oxidation-Reduction
Reductases
Transcription
Tuberculosis
Tuberculosis - microbiology
Virulence
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Summary:Both Mycobacterium tuberculosis and Mycobacterium bovis can produce tuberculosis in humans. Mycobacterium tuberculosis has low nitrate reductase activity during aerobic growth (AG), but shows strong hypoxic induction. Virulent M. bovis has weak activity during AG with no hypoxic induction. Bacille Calmette-Guerin (BCG) lacks activity in both stages. Transcription of narG of the nitrate reductase enzyme operon was higher in M. tuberculosis than in M. bovis or BCG. Transcription of narK2 encoding the nitrate transporter was induced by hypoxia in M. tuberculosis but not M. bovis or BCG. Insertion of the M. tuberculosis narGHJI operon into M. bovis resulted in increased activity only during AG. Regulation of both the nitrate reductase enzyme and transporter are regulated differently in the two species.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2008.01424.x