Loading…

Kinetic spectrofluorimetric determination of certain cephalosporins in human plasma

An accurate, reliable, specific and sensitive kinetic spectrofluorimetric method was developed for the determination of seven cephalosporin antibiotics namely cefotaxime sodium, cephapirin sodium, cephradine dihydrate, cephalexin monohydrate, cefazoline sodium, ceftriaxone sodium and cefuroxime sodi...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 2009-02, Vol.77 (4), p.1394-1404
Main Authors: Omar, Mahmoud A., Abdelmageed, Osama H., Attia, Tamer Z.
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c393t-f21c1fb9cce475c65a10d7d0b057311d88be7b964119586c2cfca61112310df73
cites
container_end_page 1404
container_issue 4
container_start_page 1394
container_title Talanta (Oxford)
container_volume 77
creator Omar, Mahmoud A.
Abdelmageed, Osama H.
Attia, Tamer Z.
description An accurate, reliable, specific and sensitive kinetic spectrofluorimetric method was developed for the determination of seven cephalosporin antibiotics namely cefotaxime sodium, cephapirin sodium, cephradine dihydrate, cephalexin monohydrate, cefazoline sodium, ceftriaxone sodium and cefuroxime sodium. The method is based on their degradation under an alkaline condition producing fluorescent products. The factors affecting the degradation and the determination were studied and optimized. The reaction is followed spectrofluorimetrically by measuring the rate of change of fluorescence intensity at specified emission wavelength. The initial rate and fixed time methods were used for the construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 0.2–1.2 μg mL −1 and 0.2–2.2 μg mL −1 using the initial rate and fixed time methods, respectively. The results were statistically validated and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. The high sensitivity of the proposed method allows the determination of investigated cephalosporins in human plasma. The statistical comparisons of the results with the reference methods show an excellent agreement and indicate no significant difference in accuracy and precision.
doi_str_mv 10.1016/j.talanta.2008.09.040
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_66738300</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0039914008006838</els_id><sourcerecordid>66738300</sourcerecordid><originalsourceid>FETCH-LOGICAL-c393t-f21c1fb9cce475c65a10d7d0b057311d88be7b964119586c2cfca61112310df73</originalsourceid><addsrcrecordid>eNqFkEtv1TAQRi1E1d5e-hNA2cAuYSbOyyuEKiiISiyAteU4Y9VXiR1sp1L_Pa5uBEtWI43ON4_D2GuECgG796cqqVm5pKoaYKhAVNDAC3bAoeclb3v-kh0AuCgFNnDFrmM8AUDNgV-yKxQwNF3bHtiPb9ZRsrqIK-kUvJk3H-xCKeTeRInCYp1K1rvCm0JTSMq6XNcHNfu4ZtbFIncetkW5Yp1VXNQrdmHUHOlmr0f26_Onn7dfyvvvd19vP96XmgueSlOjRjMKranpW921CmHqJxghX484DcNI_Si6BlG0Q6drbbTqELHmGTQ9P7J357lr8L83ikkuNmqasxbyW5Rd1_OBZwlH1p5BHXyMgYxc848qPEkE-WxTnuRuUz7blCBktplzb_YF27jQ9C-168vA2x1QUavZBOW0jX-5GnEA0TaZ-3DmKOt4tBRk1JacpsmGrF1O3v7nlD8aMpbj</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66738300</pqid></control><display><type>article</type><title>Kinetic spectrofluorimetric determination of certain cephalosporins in human plasma</title><source>ScienceDirect Journals</source><creator>Omar, Mahmoud A. ; Abdelmageed, Osama H. ; Attia, Tamer Z.</creator><creatorcontrib>Omar, Mahmoud A. ; Abdelmageed, Osama H. ; Attia, Tamer Z.</creatorcontrib><description>An accurate, reliable, specific and sensitive kinetic spectrofluorimetric method was developed for the determination of seven cephalosporin antibiotics namely cefotaxime sodium, cephapirin sodium, cephradine dihydrate, cephalexin monohydrate, cefazoline sodium, ceftriaxone sodium and cefuroxime sodium. The method is based on their degradation under an alkaline condition producing fluorescent products. The factors affecting the degradation and the determination were studied and optimized. The reaction is followed spectrofluorimetrically by measuring the rate of change of fluorescence intensity at specified emission wavelength. The initial rate and fixed time methods were used for the construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 0.2–1.2 μg mL −1 and 0.2–2.2 μg mL −1 using the initial rate and fixed time methods, respectively. The results were statistically validated and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. The high sensitivity of the proposed method allows the determination of investigated cephalosporins in human plasma. The statistical comparisons of the results with the reference methods show an excellent agreement and indicate no significant difference in accuracy and precision.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2008.09.040</identifier><identifier>PMID: 19084655</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Anti-Bacterial Agents - analysis ; Calibration ; Cephalosporins ; Cephalosporins - analysis ; Chemistry ; Chemistry, Pharmaceutical - methods ; Exact sciences and technology ; Fixed time method ; Fluorescent Dyes - pharmacology ; Humans ; Hydrogen-Ion Concentration ; Initial rate method ; Kinetic spectrofluorimetry ; Kinetics ; Models, Chemical ; Plasma ; Plasma analysis ; Reproducibility of Results ; Sodium Hydroxide - chemistry ; Spectrometric and optical methods ; Spectrometry, Fluorescence - methods ; Temperature ; Time Factors</subject><ispartof>Talanta (Oxford), 2009-02, Vol.77 (4), p.1394-1404</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-f21c1fb9cce475c65a10d7d0b057311d88be7b964119586c2cfca61112310df73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=21180954$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19084655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Omar, Mahmoud A.</creatorcontrib><creatorcontrib>Abdelmageed, Osama H.</creatorcontrib><creatorcontrib>Attia, Tamer Z.</creatorcontrib><title>Kinetic spectrofluorimetric determination of certain cephalosporins in human plasma</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>An accurate, reliable, specific and sensitive kinetic spectrofluorimetric method was developed for the determination of seven cephalosporin antibiotics namely cefotaxime sodium, cephapirin sodium, cephradine dihydrate, cephalexin monohydrate, cefazoline sodium, ceftriaxone sodium and cefuroxime sodium. The method is based on their degradation under an alkaline condition producing fluorescent products. The factors affecting the degradation and the determination were studied and optimized. The reaction is followed spectrofluorimetrically by measuring the rate of change of fluorescence intensity at specified emission wavelength. The initial rate and fixed time methods were used for the construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 0.2–1.2 μg mL −1 and 0.2–2.2 μg mL −1 using the initial rate and fixed time methods, respectively. The results were statistically validated and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. The high sensitivity of the proposed method allows the determination of investigated cephalosporins in human plasma. The statistical comparisons of the results with the reference methods show an excellent agreement and indicate no significant difference in accuracy and precision.</description><subject>Analytical chemistry</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Calibration</subject><subject>Cephalosporins</subject><subject>Cephalosporins - analysis</subject><subject>Chemistry</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>Exact sciences and technology</subject><subject>Fixed time method</subject><subject>Fluorescent Dyes - pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Initial rate method</subject><subject>Kinetic spectrofluorimetry</subject><subject>Kinetics</subject><subject>Models, Chemical</subject><subject>Plasma</subject><subject>Plasma analysis</subject><subject>Reproducibility of Results</subject><subject>Sodium Hydroxide - chemistry</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkEtv1TAQRi1E1d5e-hNA2cAuYSbOyyuEKiiISiyAteU4Y9VXiR1sp1L_Pa5uBEtWI43ON4_D2GuECgG796cqqVm5pKoaYKhAVNDAC3bAoeclb3v-kh0AuCgFNnDFrmM8AUDNgV-yKxQwNF3bHtiPb9ZRsrqIK-kUvJk3H-xCKeTeRInCYp1K1rvCm0JTSMq6XNcHNfu4ZtbFIncetkW5Yp1VXNQrdmHUHOlmr0f26_Onn7dfyvvvd19vP96XmgueSlOjRjMKranpW921CmHqJxghX484DcNI_Si6BlG0Q6drbbTqELHmGTQ9P7J357lr8L83ikkuNmqasxbyW5Rd1_OBZwlH1p5BHXyMgYxc848qPEkE-WxTnuRuUz7blCBktplzb_YF27jQ9C-168vA2x1QUavZBOW0jX-5GnEA0TaZ-3DmKOt4tBRk1JacpsmGrF1O3v7nlD8aMpbj</recordid><startdate>20090215</startdate><enddate>20090215</enddate><creator>Omar, Mahmoud A.</creator><creator>Abdelmageed, Osama H.</creator><creator>Attia, Tamer Z.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090215</creationdate><title>Kinetic spectrofluorimetric determination of certain cephalosporins in human plasma</title><author>Omar, Mahmoud A. ; Abdelmageed, Osama H. ; Attia, Tamer Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-f21c1fb9cce475c65a10d7d0b057311d88be7b964119586c2cfca61112310df73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analytical chemistry</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Calibration</topic><topic>Cephalosporins</topic><topic>Cephalosporins - analysis</topic><topic>Chemistry</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>Exact sciences and technology</topic><topic>Fixed time method</topic><topic>Fluorescent Dyes - pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Initial rate method</topic><topic>Kinetic spectrofluorimetry</topic><topic>Kinetics</topic><topic>Models, Chemical</topic><topic>Plasma</topic><topic>Plasma analysis</topic><topic>Reproducibility of Results</topic><topic>Sodium Hydroxide - chemistry</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Omar, Mahmoud A.</creatorcontrib><creatorcontrib>Abdelmageed, Osama H.</creatorcontrib><creatorcontrib>Attia, Tamer Z.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Omar, Mahmoud A.</au><au>Abdelmageed, Osama H.</au><au>Attia, Tamer Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetic spectrofluorimetric determination of certain cephalosporins in human plasma</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2009-02-15</date><risdate>2009</risdate><volume>77</volume><issue>4</issue><spage>1394</spage><epage>1404</epage><pages>1394-1404</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>An accurate, reliable, specific and sensitive kinetic spectrofluorimetric method was developed for the determination of seven cephalosporin antibiotics namely cefotaxime sodium, cephapirin sodium, cephradine dihydrate, cephalexin monohydrate, cefazoline sodium, ceftriaxone sodium and cefuroxime sodium. The method is based on their degradation under an alkaline condition producing fluorescent products. The factors affecting the degradation and the determination were studied and optimized. The reaction is followed spectrofluorimetrically by measuring the rate of change of fluorescence intensity at specified emission wavelength. The initial rate and fixed time methods were used for the construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 0.2–1.2 μg mL −1 and 0.2–2.2 μg mL −1 using the initial rate and fixed time methods, respectively. The results were statistically validated and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. The high sensitivity of the proposed method allows the determination of investigated cephalosporins in human plasma. The statistical comparisons of the results with the reference methods show an excellent agreement and indicate no significant difference in accuracy and precision.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19084655</pmid><doi>10.1016/j.talanta.2008.09.040</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0039-9140
ispartof Talanta (Oxford), 2009-02, Vol.77 (4), p.1394-1404
issn 0039-9140
1873-3573
language eng
recordid cdi_proquest_miscellaneous_66738300
source ScienceDirect Journals
subjects Analytical chemistry
Anti-Bacterial Agents - analysis
Calibration
Cephalosporins
Cephalosporins - analysis
Chemistry
Chemistry, Pharmaceutical - methods
Exact sciences and technology
Fixed time method
Fluorescent Dyes - pharmacology
Humans
Hydrogen-Ion Concentration
Initial rate method
Kinetic spectrofluorimetry
Kinetics
Models, Chemical
Plasma
Plasma analysis
Reproducibility of Results
Sodium Hydroxide - chemistry
Spectrometric and optical methods
Spectrometry, Fluorescence - methods
Temperature
Time Factors
title Kinetic spectrofluorimetric determination of certain cephalosporins in human plasma
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T20%3A12%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Kinetic%20spectrofluorimetric%20determination%20of%20certain%20cephalosporins%20in%20human%20plasma&rft.jtitle=Talanta%20(Oxford)&rft.au=Omar,%20Mahmoud%20A.&rft.date=2009-02-15&rft.volume=77&rft.issue=4&rft.spage=1394&rft.epage=1404&rft.pages=1394-1404&rft.issn=0039-9140&rft.eissn=1873-3573&rft.coden=TLNTA2&rft_id=info:doi/10.1016/j.talanta.2008.09.040&rft_dat=%3Cproquest_cross%3E66738300%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c393t-f21c1fb9cce475c65a10d7d0b057311d88be7b964119586c2cfca61112310df73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=66738300&rft_id=info:pmid/19084655&rfr_iscdi=true