Interspecies compatibility of the anther specific cell wall invertase promoters from Arabidopsis and tobacco for generating male sterile plants

• Published in: Theoretical and applied genetics 2009, Vol.118 (2), p.235-245
• Main Authors: Hirsche, J, Engelke, T, Völler, D, Götz, M, Roitsch, T
• Format: Article
• Language: English
• Subjects: Agricultural production; Agriculture; Antisense Elements (Genetics); Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - physiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Classical genetics, quantitative genetics, hybrids; Flowers - enzymology; Flowers - genetics; Fundamental and applied biological sciences. Psychology; Genes, Reporter; Genetics of eukaryotes. Biological and molecular evolution; Genomes; Germination - genetics; Glucuronidase - analysis; Life Sciences; Nicotiana - enzymology; Nicotiana - genetics; Original Paper; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - physiology; Plant reproduction; Pollen - enzymology; Pollen - genetics; Pollen - growth & development; Promoter Regions, Genetic; Pteridophyta, spermatophyta; Recombinant Fusion Proteins - analysis; Reproduction - physiology; Seeds; Species Specificity; Tobacco; Vegetals
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-008-0892-2

Histochemical GUS-staining and fluorometric analyses revealed strong tissue specific activities of the cell wall invertase promoters Nin88 from Nicotiana tabacum and AtcwINV2 from Arabidopsis thaliana that are restricted tightly to anthers and pollen, respectively. Both in A. thaliana and N. tabacum repression of invertase activity by anther specific RNA-interference turned out to be an efficient method to circumvent carbohydrate supply of the symplastically isolated pollen with subsequent strong decrease of pollen germination ability and seed setting. In the case of tobacco, comparable results were also obtained by expressing a proteinaceous invertase inhibitor, whereas this approach was less efficient in Arabidopis. The present study revealed that anther specific interference with invertase-activity in order to generate male sterile plants can be applied to members of the two different plant families Solanaceae (N. tabacum) and Brassicaceae (A. thalaina) and the strategy seems to be a general tool for practical application in hybrid breeding or as biological safety precautions. To elucidate the compatibility of the isolated promoters beyond plant families, we transferred the regulatory sequences into the respectively heterologous systems, i.e. the Nin88 promoter into Arabidopsis and the AtcwINV2 promoter into tobacco. The specificities of both promoters are maintained in the heterologous backgrounds, but their activities are strongly reduced as GUS-stainings of flowers and pollen revealed and fluorometrical quantification confirmed.