Human skin penetration of silver nanoparticles through intact and damaged skin

Abstract There is a growing interest on nanoparticle safety for topical use. The benefits of nanoparticles have been shown in several scientific fields, but little is known about their potential to penetrate the skin. This study aims at evaluating in vitro skin penetration of silver nanoparticles. E...

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Bibliographic Details
Published in:Toxicology (Amsterdam) 2009-01, Vol.255 (1), p.33-37
Main Authors: Larese, Francesca Filon, D’Agostin, Flavia, Crosera, Matteo, Adami, Gianpiero, Renzi, Nadia, Bovenzi, Massimo, Maina, Giovanni
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Damaged skin
Diffusion Chambers, Culture
Emergency
Humans
In vitro
In Vitro Techniques
Indicators and Reagents
Medical sciences
Membranes - metabolism
Metals and various inorganic compounds
Microscopy, Electron, Transmission
Nanoparticles
Silver
Silver - pharmacokinetics
Skin - chemistry
Skin - injuries
Skin - metabolism
Skin absorption
Skin Absorption - physiology
Spectrophotometry, Atomic
Tissue Fixation
Toxicology
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Summary:Abstract There is a growing interest on nanoparticle safety for topical use. The benefits of nanoparticles have been shown in several scientific fields, but little is known about their potential to penetrate the skin. This study aims at evaluating in vitro skin penetration of silver nanoparticles. Experiments were performed using the Franz diffusion cell method with intact and damaged human skin. Physiological solution was used as receiving phase and 70 μg/cm2 of silver nanoparticles coated with polyvinylpirrolidone dispersed in synthetic sweat were applied as donor phase to the outer surface of the skin for 24 h. The receptor fluid measurements were performed by electro thermal atomic absorption spectroscopy (ETAAS). Human skin penetration was also determined by using transmission electron microscope (TEM) to verify the location of silver nanoparticles in exposed membranes. Median silver concentrations of 0.46 ng cm−2 (range
ISSN:0300-483X
1879-3185
DOI:10.1016/j.tox.2008.09.025