Assessment of a dynamic hollow-fibre liquid phase microextraction system for human blood plasma samples

• Published in: Talanta (Oxford) 2009-02, Vol.77 (4), p.1309-1314
• Main Authors: Hansson, Helena, Nilsson, Ulrika
• Format: Article
• Language: English
• Subjects: Analytic Sample Preparation Methods - instrumentation; Analytic Sample Preparation Methods - methods; Analytical chemistry; Chemistry; Chromatography, Liquid - methods; Equipment Design; Exact sciences and technology; Hollow fibre; Human blood plasma; Humans; Hydrogen-Ion Concentration; Liquid phase microextraction; Mass Spectrometry - methods; Models, Chemical; Nitrophenols; Phenols - analysis; Plasma; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization - methods; Supported liquid membrane; Temperature; Time Factors; Xenobiotics - analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2008.09.005

A dynamic liquid phase microextraction (LPME) system, based on hollow-fibre supported liquid membrane (SLM) extraction, was developed for extracting ionisable xenobiotics from human plasma, and its performance was evaluated (in terms of extraction efficiency, reproducibility, durability and carry-over) using nitrophenolic compounds as model analytes at concentrations of 0.1–0.5 μg mL −1 in aqueous standards. The efficiency and repeatability were tested also on spiked human plasma. The system is non-expensive, convenient, requires minimal manual handling and enables samples with volumes as small as 0.2 mL to be extracted. For plasma samples extraction efficiencies of between 30 and 58% were achieved within 20 min, including washing steps. The limit of detection (LOD) values were in the range 0.02–0.03 μg mL −1. The developed system can provide enrichment factors up to eight, based on the injection-to-acceptor volume ratio (in this case 0.2–0.025 mL). The same hollow-fibre membrane was used up to 8 days with no loss of efficiency. Carry-over was lower than detection limit.