Dentin Regeneration by Dental Pulp Stem Cell Therapy with Recombinant Human Bone Morphogenetic Protein 2

Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin...

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Bibliographic Details
Published in:Journal of dental research 2004-08, Vol.83 (8), p.590-595
Main Authors: Iohara, K., Nakashima, M., Ito, M., Ishikawa, M., Nakasima, A., Akamine, A.
Format: Article
Language:English
Subjects:
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins - administration & dosage
Cell Differentiation - drug effects
Cells, Cultured
Culture Techniques - methods
Dental Pulp - cytology
Dental Pulp - drug effects
Dental Pulp Capping - methods
Dental Pulp Cavity - surgery
Dentin - drug effects
Dentin - physiology
Dentin - surgery
Dentinogenesis - drug effects
Dentinogenesis - physiology
Dentistry
Dogs
Humans
Odontoblasts - drug effects
Recombinant Proteins
Regeneration - drug effects
Regeneration - physiology
Stem Cell Transplantation - methods
Stem Cells - cytology
Stem Cells - drug effects
Swine
Transforming Growth Factor beta
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Summary:Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein (Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910408300802