Identification of swiprosin 1 in human lymphocytes

The aim of this work was to identify a new protein that discriminated CD8 from CD4 and CD19 lymphocytes. Proteins were separated by high‐resolution two‐dimensional electrophoresis. After silver staining, the gel images were captured with a laser densitometer, and studied with a dedicated software. T...

Full description

Saved in:
Bibliographic Details
Published in:Proteomics (Weinheim) 2004-08, Vol.4 (8), p.2216-2220
Main Authors: Vuadens, Françoise, Rufer, Nathalie, Kress, Annegret, Corthésy, Patricia, Schneider, Philippe, Tissot, Jean-Daniel
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antigens, CD19 - analysis
Calcium-Binding Proteins - analysis
Calcium-Binding Proteins - genetics
CD4 Antigens - analysis
CD8-Positive T-Lymphocytes - chemistry
CD8-Positive T-Lymphocytes - metabolism
Chromatography, Liquid
Electrophoresis, Gel, Two-Dimensional
Human blood
Humans
Lymphocytes
Mass Spectrometry
Molecular Sequence Data
Proteins - analysis
Proteins - genetics
Swiprosin 1
Two-dimensional gel electrophoresis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this work was to identify a new protein that discriminated CD8 from CD4 and CD19 lymphocytes. Proteins were separated by high‐resolution two‐dimensional electrophoresis. After silver staining, the gel images were captured with a laser densitometer, and studied with a dedicated software. This study confirmed the presence of two spots that appeared to be preferentially associated with CD8 lymphocytes, and mass spectrometry analyzes (liquid chromatography‐tandem mass spectrometry, LC‐MS/MS) identified six peptides for one spot and four for the other. The peptide sequences corresponded to an unknown protein that we named swiprosin 1 (Swiss‐Prot Q96C19). Molecular analysis (reverse transcriptase‐polymerase chain reaction, RT‐PCR) and Northern blots confirmed that the gene expression was increased in purified populations of CD8 lymphocytes, when compared to CD19 and CD4 lymphocytes. Database mining revealed that swiprosin 1 contains two potential EF‐hand domains, and therefore may have a role in calcium signaling. Its predominant presence in CD8 lymphocytes suggests that it may be involved in functions that are important for cytotoxic lymphocytes.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200300779