Development and Use of a Type-Specific Antibody Avidity Test Based on Herpes Simplex Virus Type 2 Glycoprotein G

Objectives: It is difficult to discriminate between lesions resulting from recently acquired versus established genital herpes simplex virus type 2 (HSV-2) infection. Methods not based on history or serum IgM status are needed. Goal: Our goal was to use type-specific gG-2 antibody avidity determinat...

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Bibliographic Details
Published in:Sexually transmitted diseases 2004-08, Vol.31 (8), p.508-515
Main Authors: MORROW, RHODA ASHLEY, FRIEDRICH, DAVID, KRANTZ, ELIZABETH, WALD, ANNA
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antibodies, Viral - analysis
Antibody Affinity
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay - methods
Female
General aspects
Herpes Genitalis - blood
Herpes Genitalis - diagnosis
Herpes Genitalis - pathology
Herpes simplex virus
Herpes viruses
Herpesvirus 2, Human - immunology
Herpesvirus 2, Human - isolation & purification
Human infectious diseases. Experimental studies and models
Humans
Infections
Infectious diseases
Male
Medical sciences
Medical screening
Middle Aged
Predictive Value of Tests
Recurrence
Sensitivity and Specificity
Sexually transmitted diseases
STD
Viral Envelope Proteins
Washington
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Summary:Objectives: It is difficult to discriminate between lesions resulting from recently acquired versus established genital herpes simplex virus type 2 (HSV-2) infection. Methods not based on history or serum IgM status are needed. Goal: Our goal was to use type-specific gG-2 antibody avidity determinations based on HerpeSelect HSV-2 enzyme-linked immunosorbent assay (ELISA) to identify new infections. Study: Sera (N = 168) from 71 patients with first-episode genital herpes and 45 sera from 21 patients with recurrent episodes were tested. Results: Median avidity increased from 30.2 in sera drawn ≤6 weeks to 54.9 >6 weeks after infection (P
ISSN:0148-5717
1537-4521
DOI:10.1097/01.olq.0000135993.06508.57