An innovative fixative for cytoskeletal components allows high resolution in colocalization studies using immunofluorescence techniques

Using a new fixation solution, CytoSkelFix, it is now possible to obtain superior fixation and thus resolution of cytoskeletal components using immunofluorescence and fluorescence microscopy. This fixative combines rapid cell penetration and cellular crosslinking of proteins such that both preservat...

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Bibliographic Details
Published in:Histochemistry and cell biology 2004-07, Vol.122 (1), p.1-1
Main Authors: Robinson, Rollin W, Snyder, Judith A
Format: Article
Language:English
Subjects:
Actins - analysis
Animals
Biomarkers
Cells, Cultured
Cross-Linking Reagents - chemistry
Dyneins - analysis
Fixatives
Fluorescent Antibody Technique, Indirect
Kinesin - analysis
Microtubules - chemistry
Microtubules - ultrastructure
Myosins - analysis
Potoroidae
Tissue Fixation - methods
Tubulin - analysis
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Summary:Using a new fixation solution, CytoSkelFix, it is now possible to obtain superior fixation and thus resolution of cytoskeletal components using immunofluorescence and fluorescence microscopy. This fixative combines rapid cell penetration and cellular crosslinking of proteins such that both preservation and resolution of cellular proteins can be detected. The cytoskeleton has proven very difficult to preserve, partly because of the lability of one of the filament systems (microtubules), and one single fixative is incapable of properly preserving microtubules, microfilaments, and intermediate filaments for localization in the same cell. Further, the motor proteins associated with the cytoskeletal elements are even more difficult to preserve, particularly simultaneously with the fiber system with which they associate. We present evidence that CytoSkelFix is a superior preservative and would be useful in fixation for all types of immunofluorescence colocalization studies where superior preservation is required.
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-004-0656-2