ABO blood group-incompatible living donor kidney transplantation: a prospective, single-centre analysis including serial protocol biopsies

• Published in: Nephrology, dialysis, transplantation dialysis, transplantation, 2009-01, Vol.24 (1), p.298-303
• Main Authors: Oettl, Tobias, Halter, Joerg, Bachmann, Alexander, Guerke, Lorenz, Infanti, Laura, Oertli, Daniel, Mihatsch, Michael, Gratwohl, Alois, Steiger, Juerg, Dickenmann, Michael
• Format: Article
• Language: English
• Subjects: ABO Blood-Group System; ABO incompatible; Adult; Aged; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; BK virus; Cytomegalovirus; Emergency and intensive care: renal failure. Dialysis management; Epstein-Barr virus; Female; Graft Survival; Histocompatibility Testing; Humans; immunoadsorption; Immunosorbent Techniques; Intensive care medicine; Isoantibodies - isolation & purification; Kidney Failure, Chronic - immunology; Kidney Failure, Chronic - physiopathology; Kidney Failure, Chronic - surgery; kidney transplantation; Kidney Transplantation - immunology; Kidney Transplantation - methods; Kidney Transplantation - pathology; Kidney Transplantation - physiology; Living Donors; Male; Medical sciences; Middle Aged; Prospective Studies; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the urinary system; Switzerland
• ISSN: 0931-0509; 1460-2385
• DOI: 10.1093/ndt/gfn478

Background. ABO incompatible kidney transplantation using antigen-specific immunoadsorption is increasingly performed but data on outcome, complications and protocol biopsies are still scarce. The present prospective single-centre study was aimed at these issues. Methods. This was a prospective single-centre cohort study of 10 successive ABO incompatible living donor kidney transplantations at the University Hospital Basel from September 2005 to October 2007. The following parameters were closely monitored during the whole follow-up: graft function, albuminuria, blood group antibody titres, CD19+ cell count, total IgG and IgG subclasses, CMV antigenaemia, decoy cells in the urine, EBV and polyoma BK virus PCR in the blood. Protocol biopsies were performed on Days 0 and 7 after 3, 6, 12 and 18 months. Results. Patient and graft survival is 100% after a median follow-up of 489 days (range 183–916 days). Median serum creatinine is 137 μmol/l (range 70–215 μmol/l), and median urine albumin–creatinine ratio (UACR) is 3.1 mg/ mmol (range 0.6–7.8 mg/mmol) at the time of the last follow-up. All patients had sustained diminished CD19+ cell count and/or total IgG concentrations. Neither CMV antigenaemia nor EBV replication in the blood was observed. Seven patients had positive polyoma BK virus replication in the blood but none developed polyoma virus-associated nephropathy (PVAN). Protocol biopsies revealed rejection Banff IIa in three patients on Day 7, and in one patient after 3 and 6 months. Banff Ia rejection was found in five patients. All rejection episodes resolved. Mild signs of chronic antibody-mediated rejection were observed in five patients. Conclusions. ABO-incompatible kidney transplantation seems to be successful and safe. Modifications of the current protocol may be possible and may further reduce potential side effects and costs.