Definition and system implementation of strategies for method development of chiral separations in normal- or reversed-phase liquid chromatography using polysaccharide-based stationary phases

This paper proposes strategies in normal- and reversed-phase liquid chromatography (NP-HPLC or NPLC and RP-HPLC or RPLC), which were developed using three polysaccharide-based stationary phases. Those strategies are implemented in a knowledge-based system for the chiral separation of drug enantiomer...

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Bibliographic Details
Published in:Journal of Chromatography A 2004-07, Vol.1041 (1), p.119-133
Main Authors: Matthijs, N, Perrin, C, Maftouh, M, Massart, D.L, Vander Heyden, Y
Format: Article
Language:English
Subjects:
Analysis
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Chiral stationary phases, LC
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Enantiomer separation
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General pharmacology
Knowledge-based system
Medical sciences
Method development
Pharmacology. Drug treatments
Polysaccharides
Polysaccharides - chemistry
Spectrophotometry, Ultraviolet
Stereoisomerism
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Summary:This paper proposes strategies in normal- and reversed-phase liquid chromatography (NP-HPLC or NPLC and RP-HPLC or RPLC), which were developed using three polysaccharide-based stationary phases. Those strategies are implemented in a knowledge-based system for the chiral separation of drug enantiomers. Each strategy includes a screening and an optimisation stage. The screening stage allows a fast evaluation of separation possibilities and enantioselectivity for many drugs in a short period of time, while the optimisation stage gives the opportunity to enhance, if needed, the initially obtained separation. Different examples demonstrate the effectiveness of the strategies for fast method development.
ISSN:0021-9673
DOI:10.1016/j.chroma.2004.04.040