Morphometric characteristics of choroid plexus epithelial cells in cases with significantly different psammoma bodies' presence

• Published in: Microscopy research and technique 2009-01, Vol.72 (1), p.32-41
• Main Authors: Jovanovic, Ivan, Ugrenovic, Sladjana, Vasovic, Ljiljana, Cukuranovic, Rade, Stoiljkovic, Nenad
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Aging - pathology; Atrophy - pathology; Calcinosis - pathology; Calgranulin A - analysis; Calgranulin B - analysis; Cell Size; choroid plexus; Choroid Plexus - chemistry; Choroid Plexus - pathology; Epithelial Cells - chemistry; Epithelial Cells - pathology; epithelium; Female; Humans; Image Processing, Computer-Assisted; immuno-histochemistry; Immunohistochemistry; Male; Middle Aged; morphometry; psammoma body
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.20642

Objectives: Taking into the consideration the fact that psammoma bodies have never been observed in stroma of any other organ as aging change, the aim of our research was to prove some structural similarities of choroids plexus and tumors psammoma bodies, and their possible connection with choroids plexus epithelial cells atrophy. Materials and Methods: We used 30 cadavers' right lateral ventricle central parts of choroid plexus as material. Tissue samples were routinely processed for the applied histochemical and immunohistochemical stainings. ImageJ software was used for morphometric analysis. Results: Cluster analysis showed the presence of two significantly different groups. The first group included the cases with sparse psammoma bodies and milder epithlelial atrophy with dome cells and vacuoles presence in older cases. The second group included the cases with numerous psammoma bodies and more severe epithelial atrophy, significant cystic formations and epithelial flattening presence, even in younger cases. Immunohistochemical analysis showed positive reaction of psammoma bodies and choroid plexus stroma on S100 protein. Application of S100 A8/A9 marker showed partial positive psammoma bodies' reaction and significant presence of S100 A8/A9 positive cells in choroid plexus blood vessels and stroma, especially, in cases with psammoma bodies' positive reaction on this marker. Conclusions: So, presence of more numerous psammoma bodies' might be associated with more severe choroids plexus epithelial cells atrophy. Immunohistochemical analysis showed psammoma bodies' positive reaction on S100 protein and the presence of S100 A8/A9 positive cells in stroma of cases with psammoma bodies' positive reaction on this marker. Microsc. Res. Tech., 2009. © 2008 Wiley‐Liss, Inc.