X-ray fluorescent CT imaging of cerebral uptake of stable-iodine perfusion agent iodoamphetamine analog IMP in mice

• Published in: Journal of synchrotron radiation 2009-01, Vol.16 (1), p.57-62
• Main Authors: Takeda, Tohoru, Wu, Jin, Huo, Qingkai, Yuasa, Tetsuya, Hyodo, Kazuyuki, Dilmanian, F. Avraham, Akatsuka, Takao
• Format: Article
• Language: English
• Subjects: Animals; Brain - blood supply; Brain - diagnostic imaging; Cerebral Cortex - blood supply; Cerebral Cortex - diagnostic imaging; cerebral perfusion; Cerebrovascular Circulation; fluorescent X-ray; Hippocampus - blood supply; Hippocampus - diagnostic imaging; Image Interpretation, Computer-Assisted; IMP; Iodine Isotopes; Iofetamine - pharmacokinetics; Mice; molecular imaging; Phantoms, Imaging; Thalamus - blood supply; Thalamus - diagnostic imaging; Tomography, X-Ray Computed - methods; X-ray fluorescent CT
• ISSN: 1600-5775; 0909-0495; 1600-5775
• DOI: 10.1107/S0909049508031853

Using X‐ray fluorescent computed tomography (XFCT), the in vivo and ex vivo cerebral distribution of a stable‐iodine‐labeled cerebral perfusion agent, iodoamphetamine analog (127I‐IMP), has been recorded in the brains of mice. In vivo cerebral perfusion in the cortex, hippocampus and thalamus was depicted at 0.5 mm in‐plane spatial resolution. Ex vivo XFCT images at 0.25 mm in‐plane spatial resolution allowed the visualisation of the detailed structures of these regions. The quality of the XFCT image of the hippocampus was comparable with the 125I‐IMP autoradiogram. These results highlight the sensitivity of XFCT and its considerable potential to evaluate cerebral perfusion in small animals without using radioactive agents.