Inhibition of DNA synthesis by K +-stabilised G-quadruplex promotes allelic preferential amplification

PCR preferential amplification consists of the inefficient amplification of one allele in a heterozygous sample. Here, we report the isolation of a GC-rich human minisatellite, MsH43, that undergoes allelic preferential amplification during PCR. This effect requires the existence of a (TGGGGC) 4 mot...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2004-07, Vol.571 (1), p.112-118
Main Authors: Boán, Francisco, Blanco, Miguel G, Barros, Paula, González, Ana Isabel, Gómez-Márquez, Jaime
Format: Article
Language:English
Subjects:
Base Sequence
DNA Methylation
DNA Replication - physiology
Female
Fetus
Gene Amplification
Humans
K +-dependent G-quadruplex
Liver - embryology
Liver - physiology
Male
Minisatellite MsH43
Molecular Sequence Data
Pedigree
Polymerase Chain Reaction - methods
Potassium - pharmacology
Preferential amplification
Stalling of DNA polymerization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:PCR preferential amplification consists of the inefficient amplification of one allele in a heterozygous sample. Here, we report the isolation of a GC-rich human minisatellite, MsH43, that undergoes allelic preferential amplification during PCR. This effect requires the existence of a (TGGGGC) 4 motif that is able to form a G-quadruplex in the presence of K +. This structure interferes with the DNA synthesis of the alleles harbouring this motif during PCR The present results are the first demonstration that the formation of G-quadruplex can be one of the mechanisms involved in some kinds of preferential amplification.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.06.062