Detection of Avian Polyomavirus Infection by Polymerase Chain Reaction Using Formalin-Fixed, Paraffin-Embedded Tissues

Avian polyomavirus infection in psittacines was diagnosed in tissues by the use of polymerase chain reaction (PCR) test. The tissues used in the procedure were either formalin-fixed tissues embedded in paraffin blocks or fresh tissues (heart, liver, and spleen) collected from the psittacines during...

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Bibliographic Details
Published in:Avian diseases 2004-04, Vol.48 (2), p.400-404, Article 400
Main Authors: Roy, Parimal, Dhillon, A. S, Lauerman, Lloyd, Shivaprasad, H. L
Format: Article
Language:English
Subjects:
Animals
avian polyomavirus
Aviary birds
Bird Diseases - diagnosis
Bird Diseases - virology
Birds
Birds - virology
Budgerigars
DNA Primers
DNA, Viral
Formaldehyde
formalin-fixed paraffin-embedded tissues
In Situ Hybridization - veterinary
Infections
Liver
Paraffin Embedding
pathology
Polymerase chain reaction
Polymerase Chain Reaction - veterinary
Polyomavirus
Polyomavirus Infections - diagnosis
Polyomavirus Infections - veterinary
Polyomavirus Infections - virology
Product category rules
Research Notes
Spleen
Tissue Fixation - veterinary
Viruses
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Summary:Avian polyomavirus infection in psittacines was diagnosed in tissues by the use of polymerase chain reaction (PCR) test. The tissues used in the procedure were either formalin-fixed tissues embedded in paraffin blocks or fresh tissues (heart, liver, and spleen) collected from the psittacines during necropsy. DNA was extracted from these tissues and was tested with the published primers for avian polyomavirus VP1 gene in the PCR that yielded an amplicon of 550 base pair size, which was then visualized by electrophoresis. The amplicon size was consistent with avian polyomavirus. The PCR test was found to be an effective method for identifying avian polyomavirus infection in both formalin-fixed, paraffin-embedded and fresh tissues from psittacine birds of different age groups.
ISSN:0005-2086
1938-4351
DOI:10.1637/7117