Effects of dopamine HCl on structural parameters of bovine brain membranes

Fluorescence probes located in different membrane regions were used to evaluate the effect of dopamine.HCl on the structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and thickness of the lipid bilayer) of synaptosomal plasma membrane vesicles (SPMV), w...

Full description

Saved in:
Bibliographic Details
Published in:Archives of pharmacal research 2004-06, Vol.27 (6), p.653-661
Main Authors: Bae, Moon-Kyoung, Huh, Min-Hoi, Lee, Seung-Woo, Kang, Hyun-Gu, Pyun, Jae-Ho, Kwak, Myeong-Hee, Jang, Hye-Ock, Yun, Il
Format: Article
Language:English
Subjects:
Anilino Naphthalenesulfonates
Animals
Cattle
Cell Membrane - drug effects
Cell Membrane - physiology
Cell Membrane - ultrastructure
Cerebral Cortex - ultrastructure
Dopamine - pharmacology
Fluorescent Dyes
Fluorometry
In Vitro Techniques
Lipid Bilayers
Membrane Fluidity - drug effects
Membrane Lipids - physiology
Pyrenes
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fluorescence probes located in different membrane regions were used to evaluate the effect of dopamine.HCl on the structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and thickness of the lipid bilayer) of synaptosomal plasma membrane vesicles (SPMV), which were obtained from the bovine cerebral cortex. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, and radiationless energy transfer from the tryptophan of membrane proteins to Py-3-Py and energy transfer from Py-3-Py monomers to 1-anilinonaphthalene-8-sulfonic acid (ANS) was also utilized. Dopamine.HCl increased both the bulk lateral mobility and annular lipid fluidity, and it had a greater fluidizing effect on the inner monolayer than on the outer monolayer. Furthermore, the drug had a clustering effect on membrane proteins.
ISSN:0253-6269
DOI:10.1007/BF02980166