Abnormal gene expression in extraembryonic tissue from cloned porcine embryos

The birth rate of cloned animals following somatic cell nuclear transfer (SCNT) is very low and the surviving animals have various developmental defects. We compared the morphology and transcriptional profile of extraembryonic tissue from three 26-d old SCNT pig fetuses with that from control fetuse...

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Bibliographic Details
Published in:Theriogenology 2009-01, Vol.71 (2), p.323-333
Main Authors: Chae, J.I., Lee, K.S., Kim, D.J., Han, Y.M., Lee, D.S., Lee, K.K., Koo, D.B.
Format: Article
Language:English
Subjects:
Animals
Apoptosis - physiology
Biomarkers
Blotting, Western
Cloned embryo
cloned embryos
cloning (animals)
Cloning, Organism
Cluster Analysis
embryo (animal)
Eukaryotic Initiation Factors - genetics
Eukaryotic Initiation Factors - metabolism
extraembryonic membranes
Extraembryonic Membranes - metabolism
Extraembryonic tissue
fetus
Gene Expression Profiling
gene expression regulation
Gene Expression Regulation, Developmental - genetics
microarray technology
Nuclear Transfer Techniques
nuclear transplantation
Oligonucleotide Array Sequence Analysis
Phylogeny
Somatic cell nuclear transfer
somatic cells
swine
Swine - embryology
transcription (genetics)
Transcription, Genetic
Transcriptional profiling
Up-Regulation
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Description
Summary:The birth rate of cloned animals following somatic cell nuclear transfer (SCNT) is very low and the surviving animals have various developmental defects. We compared the morphology and transcriptional profile of extraembryonic tissue from three 26-d old SCNT pig fetuses with that from control fetuses. Transcriptional profiling using long-oligonucleotide microarray technology revealed 34 genes that were differentially expressed between the three groups. The differential expression of several genes involved in translational regulation was confirmed by real-time quantitative PCR and Western blot analysis. Interestingly, the expression of a translational inhibition-related gene encoding a eukaryotic translation initiation factor 4E-binding protein was significantly elevated in the SCNT samples. We concluded that the low birth rate of cloned animals could be related to abnormal expression of translational regulators in extraembryonic tissue during early pregnancy.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2008.07.025