Soluble urokinase plasminogen activator receptor in blood transfusion components

Post‐transfusion infectious complications associated with allogeneic blood components may depend on storage time and may be related to extracellular accumulation of bioactive substances during storage. The glycoprotein, soluble urokinase plasminogen activator receptor (suPAR), which is located in sp...

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Bibliographic Details
Published in:Transfusion medicine (Oxford, England) England), 2004-08, Vol.14 (4), p.305-312
Main Authors: Riisbro, R., Brünner, N., Christensen, I. J., Nielsen, H. J.
Format: Article
Language:English
Subjects:
Blood Component Transfusion - standards
blood components
Blood Preservation - methods
blood transfusion
Cell Separation
Female
Humans
leucofiltration
Leukocytes - cytology
Male
Receptors, Cell Surface - blood
Receptors, Urokinase Plasminogen Activator
Reference Values
Software
storage time
suPAR
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Summary:Post‐transfusion infectious complications associated with allogeneic blood components may depend on storage time and may be related to extracellular accumulation of bioactive substances during storage. The glycoprotein, soluble urokinase plasminogen activator receptor (suPAR), which is located in specific granules of neutrophils, plays a role in inflammation and remodelling of the extracellular matrix. Using enzyme‐linked immunosorbent assay, suPAR was determined in serum, plasma and blood cell lysates. In addition, suPAR was measured in whole blood (WB), buffy‐coat‐depleted saline‐adenine‐glucose‐mannitol (SAGM) blood, platelet‐rich plasma (PRP) and buffy‐coat‐derived platelet (BCP) pools with and without pre‐storage leucofiltration, and in non‐filtered WB, SAGM blood and platelet concentrates prepared using apheresis (APC) at different time points during storage. Mean suPAR concentration was significantly higher in cell lysates, compared to that in a corresponding serum (P = 0·007) and in plasma samples (P = 0·004). Mean suPAR levels in WB, BCP and SAGM were significantly reduced using leucofiltration (WB: 3·4 versus 2·0 ng mL−1; BCP: 1·6 versus 1·1 ng mL−1; SAGM: 2·8 versus 0·19 ng mL−1), whereas no difference was observed in PRP. In non‐filtered WB, SAGM and APC, extracellular suPAR accumulated significantly in a storage‐time‐dependent manner (WB: P 
ISSN:0958-7578
1365-3148
DOI:10.1111/j.0958-7578.2004.00518.x