In vivo flow cytometry: A new method for enumerating circulating cancer cells

The fate of circulating tumor cells is an important determinant of their ability to form distant metastasis. Here, we demonstrate the use of in vivo flow cytometry as a powerful new method for detecting quantitatively circulating cancer cells. We specifically examine the circulation kinetics of two...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2004-08, Vol.64 (15), p.5044-5047
Main Authors: GEORGAKOUDI, Irene, SOLBAN, Nicolas, NOVAK, John, RICE, William L, XUNBIN WEI, HASAN, Tayyaba, LIN, Charles P
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Flow Cytometry - methods
Humans
Kinetics
Male
Medical sciences
Mice
Mice, SCID
Neoplasm Metastasis - diagnosis
Neoplastic Cells, Circulating - pathology
Pharmacology. Drug treatments
Prostatic Neoplasms - blood
Prostatic Neoplasms - pathology
Rats
Tumor Cells, Cultured
Tumors
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Summary:The fate of circulating tumor cells is an important determinant of their ability to form distant metastasis. Here, we demonstrate the use of in vivo flow cytometry as a powerful new method for detecting quantitatively circulating cancer cells. We specifically examine the circulation kinetics of two prostate cancer cell lines with different metastatic potential in mice and rats. We find that the cell line and the host environment affect the circulation kinetics of prostate cancer cells, with the intrinsic cell line properties determining the initial rate of cell depletion from the circulation and the host affecting cell circulation at later time points.
ISSN:0008-5472
1538-7445
DOI:10.1158/0008-5472.CAN-04-1058