Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media

• Published in: Bioresource technology 2004-12, Vol.95 (3), p.287-291
• Main Authors: Papanikolaou, Seraphim, Komaitis, Michael, Aggelis, George
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biomass; Biotechnology; carbohydrate content; cell culture; Cell Culture Techniques; culture media; Fundamental and applied biological sciences. Psychology; gamma-linolenic acid; gamma-Linolenic Acid - biosynthesis; glucose; Glucose - metabolism; industrial microbiology; lipid metabolism; lipids; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Mortierella; Mortierella - physiology; Mortierella isabellina; oils; Single cell oil; γ-Linolenic acid
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2004.02.016

Mortierella isabellina cultivated in nitrogen-limited media presented remarkable cell growth (up to 35.9 g/l) and high glucose uptake even with high initial sugar concentrations (e.g. 100 g/l) in media. After nitrogen depletion, significant fat quantities were accumulated inside the fungal mycelia (50–55%, wt/wt oil in dry biomass), resulting in a notable single cell oil production of 18.1 g/l of culture medium. Total dry biomass and lipid yields presented greatly increased values (0.34 and 0.17 g respectively per gram of glucose consumed). The microbial lipid produced contained γ-linolenic acid (GLA) at a concentration of 3.5 ± 1.0%, wt/wt, which corresponded to 16–19 mg GLA per gram of dry microbial mass and a maximum concentration of 0.801 g GLA per liter of culture medium.