Simple non-invasive assessment of advanced glycation endproduct accumulation

The accumulation of AGE is thought to play a role in the pathogenesis of chronic complications of diabetes mellitus and renal failure. All current measurements of AGE accumulation require invasive sampling. We exploited the fact that several AGE exhibit autofluorescence to develop a non-invasive too...

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Bibliographic Details
Published in:Diabetologia 2004-07, Vol.47 (7), p.1324-1330
Main Authors: MEERWALDT, R, GRAAFF, R, OOMEN, P. H. N, LINKS, T. P, JAGER, J. J, ALDERSON, N. L, THORPE, S. R, BAYNES, J. W, GANS, R. O. B, SMIT, A. J
Format: Article
Language:English
Subjects:
Adult
Age
Alzheimer's disease
Arginine - analogs & derivatives
Arginine - blood
Biological and medical sciences
Biopsy
Collagen
Diabetes
Diabetes Mellitus, Type 1 - pathology
Diabetes Mellitus, Type 2 - pathology
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinology
Endocrinopathies
Female
Fluorescence
Glycation End Products, Advanced - analysis
Glycation End Products, Advanced - metabolism
Hospitals
Humans
Lysine - analogs & derivatives
Lysine - blood
Male
Medical sciences
Medicine
Middle Aged
Patients
Proteins
Reference Values
Regression Analysis
Skin
Skin - cytology
Skin - metabolism
Skin - pathology
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Summary:The accumulation of AGE is thought to play a role in the pathogenesis of chronic complications of diabetes mellitus and renal failure. All current measurements of AGE accumulation require invasive sampling. We exploited the fact that several AGE exhibit autofluorescence to develop a non-invasive tool for measuring skin AGE accumulation, the Autofluorescence Reader (AFR). We validated its use by comparing the values obtained using the AFR with the AGE content measured in extracts from skin biopsies of diabetic and control subjects. Using the AFR with an excitation light source of 300-420 nm, fluorescence of the skin was measured at the arm and lower leg in 46 patients with diabetes (Type 1 and 2) and in 46 age- and sex-matched control subjects, the majority of whom were Caucasian. Autofluorescence was defined as the average fluorescence per nm over the entire emission spectrum (420-600 nm) as ratio of the average fluorescence per nm over the 300-420-nm range. Skin biopsies were obtained from the same site of the arm, and analysed for collagen-linked fluorescence (CLF) and specific AGE: pentosidine, N(epsilon)-(carboxymethyl)lysine (CML) and N(epsilon)-(carboxyethyl)lysine (CEL). Autofluorescence correlated with CLF, pentosidine, CML, and CEL ( r=0.47-0.62, p
ISSN:0012-186X
1432-0428
DOI:10.1007/s00125-004-1451-2