A Plant Vacuolar Protease, VPE, Mediates Virus-Induced Hypersensitive Cell Death

Programmed cell death (PCD) in animals depends on caspase protease activity. Plants also exhibit PCD, for example as a response to pathogens, although a plant caspase remains elusive. Here we show that vacuolar processing enzyme (VPE) is a protease essential for a virus-induced hypersensitive respon...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2004-08, Vol.305 (5685), p.855-858
Main Authors: Hatsugai, Noriyuki, Kuroyanagi, Miwa, Yamada, Kenji, Meshi, Tetsuo, Tsuda, Shinya, Kondo, Maki, Nishimura, Mikio, Hara-Nishimura, Ikuko
Format: Article
Language:English
Subjects:
Apoptosis
Biological and medical sciences
Capsid Proteins - metabolism
Caspase 1 - metabolism
Cell death
Cellular biology
Climate
Cysteine Endopeptidases - genetics
Cysteine Endopeptidases - metabolism
DNA Fragmentation
DNA, Plant - metabolism
Endopeptidases - genetics
Endopeptidases - metabolism
Enzymes
Evidence
Flowers & plants
Fundamental and applied biological sciences. Psychology
Gene Silencing
Hypersensitivity
Intracellular Membranes - ultrastructure
Leaves
Lesions
Nicotiana
Nicotiana - cytology
Nicotiana - enzymology
Nicotiana - genetics
Nicotiana - virology
Pathogens
Pathology. Damages, economic importance
Physiological aspects
Phytopathology. Animal pests. Plant and forest protection
Plant cells
Plant Diseases - virology
Plant Leaves - cytology
Plant Leaves - enzymology
Plant Leaves - virology
Plant physiology
Plant Proteins - metabolism
Plant viruses and viroids
Plants
Protease Inhibitors - pharmacology
Proteases
Protoplasts
Protoplasts - ultrastructure
Temperature
Tobacco
Tobacco Mosaic Virus - physiology
Vacuoles
Vacuoles - enzymology
Vacuoles - ultrastructure
Virus Replication
Viruses
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Description
Summary:Programmed cell death (PCD) in animals depends on caspase protease activity. Plants also exhibit PCD, for example as a response to pathogens, although a plant caspase remains elusive. Here we show that vacuolar processing enzyme (VPE) is a protease essential for a virus-induced hypersensitive response that involves PCD. VPE deficiency prevented virus-induced hypersensitive cell death in tobacco plants. VPE is structurally unrelated to caspases, although VPE has a caspase-1 activity. Thus, plants have evolved a regulated cellular suicide strategy that, unlike PCD of animals, is mediated by VPE and the cellular vacuole.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1099859