The Identification of Three Human Metabolites of a Peptide-Doxorubicin Conjugate Using HPLC-MS-MS in Positive and Negative Ionization Modes

A peptide-doxorubicin conjugate (I) is a drug candidate that has been evaluated for the treatment of prostate cancer. During the high-performance liquid chromatographic (HPLC)-fluorescence analysis of clinical samples for compound I and its two known metabolites [doxorubicin (II) and leucine-doxorub...

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Bibliographic Details
Published in:Journal of chromatographic science 2004-07, Vol.42 (6), p.317-322
Main Authors: Desai, R.B., Schwartz, M.S., Matuszewski, B.K.
Format: Article
Language:English
Subjects:
Analysis
Antibiotics, Antineoplastic - chemistry
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Doxorubicin - chemistry
General pharmacology
Humans
Mass Spectrometry - methods
Medical sciences
Peptides - chemistry
Pharmacology. Drug treatments
Spectrometry, Fluorescence
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Summary:A peptide-doxorubicin conjugate (I) is a drug candidate that has been evaluated for the treatment of prostate cancer. During the high-performance liquid chromatographic (HPLC)-fluorescence analysis of clinical samples for compound I and its two known metabolites [doxorubicin (II) and leucine-doxorubicin (III)], additional metabolites are observed in postdose human plasma extracts. Using neutral loss, precursor, and product ion tandem mass spectrometric (MS-MS) experiments, two of these metabolites are identified as doxorubicinol (IV) and leucine-doxorubicinol (V), the active 13-hydroxy metabolites of doxorubicin and leucine-doxorubicin, respectively. A third metabolite, 7-deoxydoxorubicinol aglycone (VI), is detected using single-ion monitoring at m/z 399 in the negative ionization mode. The product ion mass spectrum of this metabolite contains a major fragment at m/z 351, resulting from the loss of water and formaldehyde from the pseudomolecular ion. An HPLC-MS-MS method for simultaneous analysis of II, III, IV, V, and VI is developed utilizing gradient HPLC with a combination of positive/negative ionization MS in the multiple reaction monitoring mode and monitoring the appropriate MS-MS transitions. Using this methodology, rat, dog, and human plasma metabolite profiles are compared and found to be qualitatively similar. Simultaneous fluorescence and MS detection experiments confirm that the peaks observed in the HPLC-fluorescence chromatograms of plasma extracts correspond to each of the five metabolites (II–VI).
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/42.6.317