Effects of Tamm–Horsfall protein on polymorphonuclear leukocyte function

Background. Tamm–Horsfall protein (THP), a glycoprotein produced exclusively by renal tubular cells, is thought to be involved in several inflammatory disorders such as bacterial interstitial nephritis as well as in defence against uropathogenic microorganisms. The specific effects of THP on inflamm...

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Bibliographic Details
Published in:Nephrology, dialysis, transplantation dialysis, transplantation, 2004-09, Vol.19 (9), p.2192-2197
Main Authors: Wimmer, Thomas, Cohen, Gerald, Saemann, Marcus D., Hörl, Walter H.
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Adult
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
apoptosis
Apoptosis - drug effects
Biological and medical sciences
chemotaxis
Chemotaxis, Leukocyte - drug effects
Emergency and intensive care: renal failure. Dialysis management
Female
Humans
In Vitro Techniques
Intensive care medicine
Male
Medical sciences
Middle Aged
Mucoproteins - pharmacology
Nephrology. Urinary tract diseases
Neutrophils - drug effects
Neutrophils - physiology
phagocytosis
Phagocytosis - drug effects
Pharmacology. Drug treatments
polymorphonuclear leukocytes
Tamm–Horsfall protein
Urinary lithiasis
Urinary system
Uromodulin
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Summary:Background. Tamm–Horsfall protein (THP), a glycoprotein produced exclusively by renal tubular cells, is thought to be involved in several inflammatory disorders such as bacterial interstitial nephritis as well as in defence against uropathogenic microorganisms. The specific effects of THP on inflammatory cells, however, are not yet well known. Therefore, the present study investigates the effects of THP in its soluble form on distinct polymorphonuclear leukocyte (PMNL) functions. Methods. PMNL were isolated from the venous blood of healthy adult donors and incubated at low THP concentrations (70–350 ng/ml), resembling plasma concentrations, and at high THP concentrations (1.75–8.75 µg/ml), resembling urinary concentrations. Results. High (urinary) THP concentrations inhibited PMNL apoptosis and chemotaxis and stimulated PMNL phagocytosis, while low (plasma) THP concentrations increased PMNL chemotaxis. Conclusions. These data indicate that THP influences several PMNL functions, suggesting a crucial immunomodulatory role for this glycoprotein in host defence mechanisms of the kidney and genitourinary tract.
ISSN:0931-0509
1460-2385
DOI:10.1093/ndt/gfh206