Detection of antibodies against a human papillomavirus (HPV) type 16 peptide that differentiate high-risk from low-risk HPV-associated low-grade squamous intraepithelial lesions

• Published in: Journal of general virology 2004-09, Vol.85 (9), p.2643-2650
• Main Authors: Rocha-Zavaleta, Leticia, Ambrosio, Juana P, Mora-Garcia, Maria de Lourdes, Cruz-Talonia, Fernando, Hernandez-Montes, Jorge, Weiss-Steider, Benny, Ortiz-Navarrete, Vianney, Monroy-Garcia, Alberto
• Format: Article
• Language: English
• Subjects: Adult; Antibodies, Viral - blood; Biological and medical sciences; Biomarkers, Tumor - blood; Capsid Proteins - immunology; Cervical Intraepithelial Neoplasia - blood; Cervical Intraepithelial Neoplasia - diagnosis; Cervical Intraepithelial Neoplasia - immunology; Cervix Uteri - immunology; Cervix Uteri - metabolism; DNA, Viral - analysis; Enzyme-Linked Immunosorbent Assay; Female; Fundamental and applied biological sciences. Psychology; Human papillomavirus; Humans; Immunoglobulin A - analysis; Immunoglobulin G - blood; Microbiology; Middle Aged; Miscellaneous; Oncogene Proteins, Viral - immunology; Papillomaviridae - classification; Papillomaviridae - immunology; Papillomaviridae - isolation & purification; Papillomavirus Infections - blood; Papillomavirus Infections - diagnosis; Papillomavirus Infections - immunology; Polymerase Chain Reaction; Risk Factors; Sensitivity and Specificity; Uterine Cervical Neoplasms - blood; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - immunology; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/vir.0.80077-0

1 Department of Molecular Biology and Biotechnology, Institute of Biomedical Research, National University of Mexico, Circuito Escolar S/N, Cd Universitaria, Apdo Postal 70228, DF CP 04510 Mexico City, Mexico 2 Laboratory of Immunobiology (L-326), Unit of Research on Cellular Differentiation and Cancer, FES Zaragoza, National University of Mexico, Mexico City, Mexico 3 National Center for Clinics of Dysplasias (CENACLID), General Hospital of Mexico, Mexico City, Mexico 4 Unit of Molecular Biomedicine, CINVESTAV, IPN, Mexico City, Mexico Correspondence Alberto Monroy-Garcia albertomon{at}yahoo.com A nonapeptide (16L1) was derived from the human papillomavirus type 16 (HPV-16) major capsid protein and tested for detection of potential cross-reactive serum IgG and cervical IgA antibodies in low- and high-risk HPV-associated low-grade squamous intraepithelial lesions (LSIL) and cervical cancer patients by ELISA. The IgG response was similar in women with low-risk HPV-associated LSIL and controls ( P =0·1). In contrast, more than 90 % of patients with high-risk HPV-associated LSIL were seropositive. Although tumours from cancer patients were all positive for the presence of high-risk HPV DNA, the level of seropositivity decreased significantly in this group ( P