Cells synchronized in S phase show increased rate of repair of UV damaged plasmids

The capacity for nucleotide excision repair of cells synchronized in S phase and unsynchronized cells was compared by the host cell reactivation assay and the cell‐free repair system. HeLa cells were transfected with in vitro damaged by UV irradiation pEGFP and the repair capacity was determined by...

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Bibliographic Details
Published in:FEBS letters 2004-08, Vol.572 (1-3), p.99-102
Main Authors: Gospodinov, Anastas, Anachkova, Boyka
Format: Article
Language:English
Subjects:
Cell Cycle - radiation effects
Cell Line
Cell-free repair system
DNA Repair - radiation effects
DNA, Neoplasm - radiation effects
Host cell reactivation
Humans
K562 Cells
Kinetics
Nucleotide excision repair
Plasmids - radiation effects
S phase
S Phase - radiation effects
Ultraviolet Rays
Xeroderma Pigmentosum
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Summary:The capacity for nucleotide excision repair of cells synchronized in S phase and unsynchronized cells was compared by the host cell reactivation assay and the cell‐free repair system. HeLa cells were transfected with in vitro damaged by UV irradiation pEGFP and the repair capacity was determined by the number of fluorescent cells. In the cell‐free repair system, the repair capacity of protein extracts isolated from K562 cells was determined by measuring the transformation efficiency of UV irradiated pBlueScript incubated in the extracts. In both cases, the repair capacity of the cells synchronized in S phase cells was 30–50% higher than the repair capacity of unsynchronized cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.07.015